In this study, gene silencing with double-stranded RNA (dsRNA) and microarray techniques were used to study the biological function of the cAMP-dependent protein kinase catalytic subunit (PfPKAc) in the parasite erythrocytic life cycle.
Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. The leaf acetone, ethyl acetate, and methanol extracts of Aegle marmelos (Linn.) Correa ex Roxb, Andrographis lineata Wallich ex Nees, and Cocculus hirsutus (L.) Diels were tested for oviposition-deterrent, ovicidal, and repellent activities against Anopheles subpictus Grassi (Diptera: Culicidae).
Allelic discrimination assays have traditionally been applied to study zygosity in diploid genomes. The assays presented here are instead successfully applied to the identification and evaluation of transcriptional activity of duplicated genes in the haploid genome of the P. falciparum parasite.
The paper compares the sequences of LSA-3 from four geographical areas and found LSA-3 to be a highly conserved antigen. This finding further supports the usefulness of LSA-3 as a pre-erythrocytic stage vaccine candidate.
The behaviour of providers is often influenced by their knowledge, financial incentives, competition, or to follow patient demands and expectations. This study used a pre-tested structured questionnaire to collect data from 225 providers from hospitals and non-hospitals about their malaria treatment practices.
The clinical presentation of pregnancy-associated malaria depends crucially on the particular epidemiological settings. By re-examining historical data, and the use of a simple mathematical model, it is demonstrated how excess female mortality can be used to evaluate the burden of pregnancy-associated malaria.
An interesting paper which addresses the issue of delayed post-mortem diagnosis of P. falciparum with ICT tests.
This review on access to malaria treatment in Kenya is particularly interesting in the light of the wealth of studies that have been carried out on related topics in Kenya.
Failure to distinguish between individuals who do not get a clinical episode during follow-up because they were unexposed and those who are genuinely immune undermines our ability to assign a protective role to immune responses against malaria. The brevity of antibodies responses makes it difficult to assign the true serological status of an individual at any given time, i.e. those positive at a survey may be negative by the time they encounter the next infection.
In areas where non-falciparum malaria is common rapid diagnostic tests (RDTs) capable of distinguishing malaria species reliably are needed. Such tests are often based on the detection of parasite lactate dehydrogenase (pLDH). In Dawei, southern Myanmar, three pLDH based RDTs (CareStartTM Malaria pLDH (Pan), CareStartTM Malaria pLDH (Pan, Pf) and OptiMAL-IT(R)) were evaluated in patients presenting with clinically suspected malaria.