Methodology: A protocol for membrane feeding assays to determine the infectiousness of P. falciparum naturally infected individuals to Anopheles gambiae

Author(s):

André Lin Ouédraogo, Wamdaogo M Guelbéogo, Anna Cohuet, Isabelle Morlais, Jonas G. King, Bronner P. Gonçalves, Guido J. H. Bastiaens, Michiel Vaanhold, Jetsumon Sattabongkot, Yimin Wu, Mamadou Coulibaly, Baber Ibrahima, Sophie Jones, Merribeth Morin, Chris Drakeley, Rhoel R. Dinglasan, Teun Bousema

Reference:

MWJ 2013, 4, 16

Access:

Open Access 2.0

Article type:

Methodology

Abstract:

Mosquito feeding assays play an important role in quantifying malaria transmission potential in epidemiological and clinical studies. At present, membrane feeding assays are incompletely standardised. This affects our understanding of the precision of the assay and its suitability for evaluating transmission-blocking interventions. Here, we present a detailed protocol for membrane feeding using Anopheles gambiae mosquitoes and naturally P. falciparum infected individuals. A seperate supplement to this article provides a detailed protocol for undertaking membrane feeding assays.