Reply to: Loop-Mediated Isothermal Amplification Method (LAMP): Low and Effective cost Novel Tool for Molecular Public Health
Hello I am trying to perform LAMP for the identification of plant fungal pathogen. Used Bst pol 2 protocol with and without betaine at 65C but did not get any product. I used 4 different sets of primers. However, when I used F3 and B3 primer got amplification of target gene by PCR (annealing was at 54C) by all the four sets of primers. Any suggestion to develop LAMP in this case?
Reply to: How to explain the high solubility of artemisinin in Artemisia annua infusions?
To clarify, I think I am to understand that higher concentrations of potassium can often be found in the stems vs. the leaves but levels of artemisinin is not at a higher concentration in stems or leaves per-say.
Reply to: Obliterated, erased. Bhy who? by WHO?
I have likewise noticed (thrice) the apparent disappearance of published articles from cyberspace. For instance, something I wrote went missing from "Trends in Parasitology" (2001, Immune response to Trichuris trichiura, 171 (3; March): 121) in that it was not listed online. Therefore, it could not be accessed via the Internet, despite being in the hard copy version of the journal. It was as if the contribution did not exist. Last year, the matter was drawn to the attention of Elsevier, and I note that the publication has become reincarnated. It is now listed amongst the journal issue's contents. The same applies to Mueller et al.'s 2000 paper in the "Journal of Ethnopharmacology", cited in Pierre Lutgen's blog (I have not looked for any of the other articles referred to there), so perhaps the problem was mentioned to the publisher (also Elsevier) and they have in the meantime fixed it.
Reply to: Should Artemisia annua (wormwood) tea be used as a prophylactic in endemic countries?
Yes for high risk groups this could be a very useful and practical means of reducing severe malaria cases and deaths, at the same time as avoiding inferior quality and fake drugs which are constantly finding their way into the market.
Reply to: Health worker and policy-maker perspectives on use of intramuscular artesunate for pre-referral and definitive treatment of severe malaria at health posts in Ethiopia
HI all, you raise a very important point; health centres/posts are usually not the most suitable places to treat severe malaria. A pre-referral dose is than preferred. However, you also mention definite treatment for severe malaria when referral is not possible, or delayed. I think we should think together what the best treatment is in this case, and if that should always be combined with antibiotics, for lack of diagnostic possibilities in a health centre. A more formal point of view of this "less desired, but in reality necessary treatment of severe malaria in Health Centres or Health Posts" is very welcome.