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in vitro

Short Technical Report: Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment

April 11, 2011 - 15:03 -- Patrick Sampao
Author(s): 
Voravuth Somsak, Somdet Srichairatanakool, Sumalee Kamchonwongpaisan, Yongyuth Yuthavong, Chairat Uthaipibull
Reference: 
Molecular and Biochemical Parasitology, Volume 177, Issue 2, June 2011, Pages 156-159

This small-scale protocol provides significant advantages, namely reduction of parasite sample, laboratory consumables and mice for transfection experiments.

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In Vitro Generation of Plasmodium falciparum Ookinetes

December 3, 2010 - 07:12 -- Patrick Sampao
Author(s): 
Viengngeun Bounkeua, Fengwu Li, AND Joseph M. Vinetz
Reference: 
Am J Trop Med Hyg, Dec 2010; 83: 1187 - 1194.

This report details methods to optimize production of P. falciparum sexual stage parasites, including ookinetes. Flow cytometric sorting was used to separate diploid/tetraploid zygotes and ookinetes from haploid gametetocytes and unfertilized gametes based on DNA content.

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Brief Report: Optimized In Vitro Production of Plasmodium vivax Ookinetes

December 2, 2010 - 12:56 -- Patrick Sampao
Author(s): 
Colleen M. McClean, Haydee Guerra Alvarado, Victor Neyra, Alejandro Llanos-Cuentas, and Joseph M. Vinetz
Reference: 
Am J Trop Med Hyg, Dec 2010; 83: 1183 - 1186.

Here, we report protocols that produce improved P. vivax ookinete yields and morphological development.

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Open Access | Methodology: Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays

June 7, 2010 - 12:05 -- Patrick Sampao
Author(s): 
Wilson DW, Crabb BS, Beeson JG
Reference: 
Malaria Journal 2010, 9:152 (3 June 2010)

Flow cytometry based assays using GFP-fluorescent parasites proved sensitive and highly reproducible for quantifying the growth-inhibitory activity of antibodies and anti-malarials, with superior reproducibility to light microscopy, and are suitable for high-throughput applications.

In Vitro Sensitivities of Plasmodium falciparum to Different Antimalarial Drugs in Uganda

February 25, 2010 - 13:51 -- Kabogo Ndegwa
Author(s): 
Samuel L. Nsobya, Moses Kiggundu, Sarah Nanyunja, Moses Joloba, Bryan Greenhouse, and Philip J. Rosenthal
Reference: 
Antimicrobial Agents and Chemotherapy, March 2010, Pages 1200-1206, Volume 54, No. 3

The control of malaria is challenged by resistance of Plasmodium falciparum to multiple drugs. New combination regimens are now advocated for the treatment of uncomplicated falciparum malaria, but the extent of resistance to newer agents is incompletely understood.

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In vitro sensitivity of Plasmodium falciparum to conventional and novel antimalarial drugs in Papua New Guinea

February 11, 2010 - 13:36 -- Kabogo Ndegwa
Author(s): 
Rina P. M. Wong, Dulcie Lautu, Livingstone Tavul, Sara L. Hackett, Peter Siba, Harin A. Karunajeewa, Kenneth F. Ilett, Ivo Mueller, Timothy M. E. Davis
Reference: 
Tropical Medicine & International Health, Volume 15, Issue 3, March 2010, Pages: 342-349

To facilitate the monitoring of antimalarial drug resistance in this setting, we assessed the in vitro sensitivity of Plasmodium falciparum isolates from Madang Province.

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A new series of amodiaquine analogues modified in the basic side chain with in vitro antileishmanial and antiplasmodial activity

November 26, 2009 - 06:41 -- Patrick Sampao
Author(s): 
Stefano Guglielmo, Massimo Bertinaria, Barbara Rolando, Marco Crosetti, Roberta Fruttero, Vanessa Yardley, Simon L. Croft, Alberto Gasco
Reference: 
European Journal of Medicinal Chemistry, Volume 44, Issue 12, December 2009, Pages 5071-5079, doi:10.1016/j.ejmech.2009.09.012

The synthesis and the study of new amodiaquine derivatives bearing modified lateral basic chains as new agents with both antimalarial and antileishmanial activities are reported.

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