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multiplex PCR

The development and evaluation of a single step multiplex PCR for simultaneous detection of Anopheles annularis group mosquitoes, human host preference and Plasmodium falciparum sporozoite presence

October 25, 2009 - 17:45 -- Bart G.J. Knols
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Author(s): 
Sunita Swain, Amitav Mohanty, Namita Mahapatra, S.K. Parida, N.S. Marai, H.K. Tripathy, Santanu K. Kar, Rupenangshu K. Hazra
Reference: 
Transactions of the Royal Society of Tropical Medicine and Hygiene, Volume 103, Issue 11, November 2009, Pages 1146-1152, doi:10.1016/j.trstmh.2009.03.022

The Anopheles annularis group mosquitoes, subgenus Cellia Theobald (Diptera: Culicidae), includes five recognized species: An. annularis Van der Wulp, An. nivipes Theobald, An. pallidus Theobald, An. philippinensis Ludlow and An. schueffneri Stanton. From these five, the three most common species found in Orissa were considered for this study because of their remarkable vectorial and behavioral variation and the important role they play in malaria transmission. To identify and understand their role in malaria transmission we developed a single multiplex PCR-based assay.  This one-step PCR-based method constitutes a very powerful tool in large surveys of anopheline populations.

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