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LAMP

Parasitology: Mitochondrial DNA Targets Increase Sensitivity of Malaria Detection Using Loop-Mediated Isothermal Amplification

July 30, 2010 - 12:31 -- Patrick Sampao
Author(s): 
Spencer D. Polley, Yasuyoshi Mori, Julie Watson, Mark D. Perkins, Iveth J. González, Tsugunori Notomi, Peter L. Chiodini, and Colin J. Sutherland
Reference: 
J. Clin. Microbiol., Aug 2010; 48: 2866 - 2871.

Here we describe the use of mitochondrial targets for LAMP-based detection of any Plasmodium genus parasite and of P. falciparum specifically.

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Comparative Diagnosis of Malaria Infections by Microscopy, Nested PCR, and LAMP in Northern Thailand

July 2, 2010 - 12:54 -- Patrick Sampao
Author(s): 
Birgit Pöschl, Jarurin Waneesorn, Oriel Thekisoe, Salakchit Chutipongvivate, AND Karanis Panagiotis
Reference: 
Am J Trop Med Hyg, Jul 2010; 83: 56 - 60.

This study further validates LAMP as an alternative molecular diagnostic tool, which can be used in the diagnosis of early infections of malaria cases and together with nPCR can also be used as supplementary methods for clinical and epidemiological use.

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Loop-Mediated Isothermal Amplification (LAMP) for Rapid Identification of Anopheles gambiae and Anopheles arabiensis Mosquitoes

December 18, 2009 - 16:41 -- Ingeborg van Schayk
Author(s): 
Mariangela Bonizzoni, Yaw Afrane, AND Guiyun Yan
Reference: 
Am J Trop Med Hyg 2009;81 1030-1034

The LAMP-based species identification method can be performed in a water bath and completed within 65 minutes, representing an alternative method for rapid and field applicable vector species diagnosis.

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