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microscopy

Research: Assessment of competence of participants before and after 7-day intensive malaria microscopy training courses in Nigeria

June 9, 2015 - 11:36 -- Bart G.J. Knols
Author(s): 
Yetunde A. Olukosi, Chimere O. Agomo, Oluwagbemiga O. Aina, Samuel K. Akindele, Hilary I. Okoh, Margaret O. Akinyele, Olusola Ajibaye, Bassey A. Orok, Bamidele A. Iwalokun, Veronica Enya, Uche T. Igbasi, Samson Awolola
Reference: 
MWJ2015, 6, 6
 
Accuracy of malaria diagnosis by microscopy has been a challenge in Nigeria due to poor competence of microscopists and inability to report on malaria species other than Plasmodium falciparum. Short courses were conducted to improve the skills of laboratory personnel to perform malaria microscopy in public health facilities. Seven-day malaria microscopy courses were conducted annually between 2011 and 2013.The training courses contained theoretical and practical sessions. Impact of the training was evaluated by practical and theoretical pre- and post-training assessments on malaria slide reading, parasite enumeration and basic malariology. The 102 participants who completed the training consisted of medical laboratory scientists (62; 60.8%), medical laboratory technicians (24; 23.5%) and other healthcare workers (16; 15.7%). The knowledge of basic malariology (theory) at pre- and post-tests were 34% (95% CI 31.7-36.3%) and 74.9% (95% CI 71.8-78.0%), respectively (P<0.001). The mean slide reading detection, species and counting agreements in pre-training assessment were 48.9%, 27.9% and 0%, respectively, and in post-training 56.8%, 39.2% and 25%, respectively. The mean species agreements in picture test pre- and post-training were 21.9% and 55.1%, respectively. There were significant differences (P<0.05) in the median pre-test scores in picture tests and basic malariology of the three categories of participants but not in malaria slide reading and parasite counting tests. However, post-training, a significant difference in test scores of the three categories of participants was recorded only for basic malariology (P=0.0003). The 7-day malaria microscopy courses significantly increased the knowledge and microscopy skills of the trainees and were sufficient to bridge the significant difference in baseline microscopy skills of the different categories of trainees that participated in the training courses.

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Usefulness of Polymerase Chain Reaction to Supplement Field Microscopy in a Pre-Selected Population with a High Probability of Malaria Infections

July 7, 2011 - 12:58 -- Patrick Sampao
Author(s): 
W. M. Kumudunayana T. de A. W. Gunasekera, Rabindra R. Abeyasinghe, Sunil Premawansa and S. Deepika Fernando
Reference: 
Am J Trop Med Hyg 2011 vol. 85 no. 1 6-11

This study determines the use of nested PCR as a diagnostic tool to supplement field microscopy in symptomatic individuals suspected of being positive for malaria, and it explores its role in active case detection to identify asymptomatic parasite carriers.

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Diagnosis of Gestational, Congenital, and Placental Malaria in Colombia: Comparison of the Efficacy of Microscopy, Nested Polymerase Chain Reaction, and Histopathology

June 2, 2011 - 14:56 -- Patrick Sampao
Author(s): 
Ivón M. Campos, Mary L. Uribe, Carolina Cuesta, Alexander Franco-Gallego, Jaime Carmona-Fonseca, and Amanda Maestre
Reference: 
Am J Trop Med Hyg 2011 84:929-935

We designed a study to compare the technical and the operational-economical performances of light microscopy (LM), nested polymerase chain reaction (nPCR), and histopathology (HP).

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Evaluation of a Comprehensive Refresher Training Program in Malaria Microscopy Covering Four Districts of Uganda

May 4, 2011 - 15:03 -- Patrick Sampao
Author(s): 
Moses Kiggundu, Samuel L. Nsobya, Moses R. Kamya, Scott Filler, Sussan Nasr, Grant Dorsey, AND Adoke Yeka
Reference: 
Am J Trop Med Hyg, May 2011; 84: 820 - 824.

To improve capacity for malaria microscopy in Uganda, a 3-day refresher training program was conducted in four districts. Training impact was measured through a written examination and evaluation of the quality of blood-slide preparation and accuracy of field microscopy.

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Congenital Malaria in Calabar, Nigeria: The Molecular Perspective

March 2, 2011 - 12:36 -- Patrick Sampao
Author(s): 
Olabisi A. Oduwole., G. C. Ejezie.,Martin Meremikwu., et al.
Reference: 
Am J Trop Med Hyg, Mar 2011; 84: 386 - 389.

We estimated the prevalence of congenital malaria using nested PCR amplification of the small subunit 18S RNA gene to detect low-level parasitemia and identify Plasmodium species in 204 mother–neonate pairs. Cord-blood parasitemia was detected in four babies by PCR, giving a prevalence of 2.0%.

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Comparative Diagnosis of Malaria Infections by Microscopy, Nested PCR, and LAMP in Northern Thailand

July 2, 2010 - 12:54 -- Patrick Sampao
Author(s): 
Birgit Pöschl, Jarurin Waneesorn, Oriel Thekisoe, Salakchit Chutipongvivate, AND Karanis Panagiotis
Reference: 
Am J Trop Med Hyg, Jul 2010; 83: 56 - 60.

This study further validates LAMP as an alternative molecular diagnostic tool, which can be used in the diagnosis of early infections of malaria cases and together with nPCR can also be used as supplementary methods for clinical and epidemiological use.

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Open Access | Multiplex real-time quantitative PCR, microscopy and rapid diagnostic immuno-chromatographic tests for the detection of Plasmodium spp: performance, limit of detection analysis and quality assurance

December 14, 2009 - 11:40 -- Ingeborg van Schayk
Author(s): 
Krishna Khairnar, Donald Martin, Rachel Lau, Filip Ralevski, Dylan R Pillai
Reference: 
Malaria Journal 2009, 8:284 (9 December 2009)

Multiplex QPCR but not ICTs are an essential adjunct to microscopy in the reference laboratory detection of malaria species specifically due to the superior LOD.

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