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PCR

Parasitology: Comparison of the Novel Partec Rapid Malaria Test to the Conventional Giemsa Stain and the Gold Standard Real-Time PCR

July 30, 2010 - 12:18 -- Patrick Sampao
Author(s): 
Bernard N., Alex A., et al.
Reference: 
J. Clin. Microbiol., Aug 2010; 48: 2925 - 2928.

In this study, we evaluated the recently developed Partec rapid malaria test (PM) for the detection of Plasmodium spp. in human blood from patients in an area where malaria is endemic and compared the results with those of thick blood film Giemsa stain (GS) in terms of its performance and operational characteristics, using real-time (RT) PCR as the gold standard.

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Open Access | Methodology: Rapid identification of chloroquine and atovaquone drug resistance in Plasmodium falciparum using high-resolution melt polymerase chain reaction

May 24, 2010 - 13:02 -- Kabogo Ndegwa
Author(s): 
Gan L, Loh J
Reference: 
Malaria Journal 2010, 9:134 (21 May 2010)

PCR-HRM is an inexpensive option for the determination of drug resistance profile in P. falciparum and will see increasing use as an alternative to sequencing and 5'nuclease PCR assays in reference laboratories or once PCR systems that are able to conduct HRM become commonplace.

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Open Access | Short Report: Evaluation of three PCR-based diagnostic assays for detecting mixed Plasmodium infection

April 8, 2010 - 07:42 -- Kabogo Ndegwa
Author(s): 
T Mixson-Hayden, NW Lucchi, V Udhayakumar
Reference: 
BMC Res Notes (2010) 3: 88

The nested PCR method remains the method of choice for the detection of mixed malaria infections and especially of sub-clinical infections. 

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Monitoring of failure of chloroquine treatment for Plasmodium vivax using polymerase chain reaction in Sanliurfa province, Turkey

March 17, 2010 - 10:46 -- Ingeborg van Schayk
Author(s): 
Fuat Dilmec, M. Ali Kurcer, Feridun Akkafa & Zeynep Simsek
Reference: 
Parasitology Research, Volume 106, Number 4 / March, 2010 : 783-788

Our target was to determine the Plasmodium species in the southeast region of Turkey and the therapeutic efficacy of CQ used in the treatment of malaria.

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Opinion: The perils of PCR: can we accurately ‘correct’ antimalarial trials?

February 26, 2010 - 10:57 -- Kabogo Ndegwa
Author(s): 
Jonathan J. Juliano, Nahla Gadalla, Colin J. Sutherland, Steven R. Meshnick
Reference: 
Trends in Parasitology, Volume 26, Issue 3, March 2010, Pages 119-124

During follow-up in antimalarial drug trials, treated subjects can be newly infected. PCR correction is used to distinguish this re-infection from drug failure (recrudescence) and to adjust final drug efficacy estimates.

Case Report: Molecular method for the diagnosis of imported pediatric malaria

February 19, 2010 - 12:16 -- Kabogo Ndegwa
Author(s): 
L. Delhaes Jeanne, A. Berry, E. Dutoit, F. Leclerc, J. Beaudou, S. Leteurtre, D. Camus, F. Benoit-Vical
Reference: 
Médecine et Maladies Infectieuses, Volume 40, Issue 2, February 2010, Pages 115-118

We report a case of imported malaria in a boy, illustrating the epidemiological and clinical aspects of severe pediatric malaria.

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Open Access | Multiplex real-time quantitative PCR, microscopy and rapid diagnostic immuno-chromatographic tests for the detection of Plasmodium spp: performance, limit of detection analysis and quality assurance

December 14, 2009 - 11:40 -- Ingeborg van Schayk
Author(s): 
Krishna Khairnar, Donald Martin, Rachel Lau, Filip Ralevski, Dylan R Pillai
Reference: 
Malaria Journal 2009, 8:284 (9 December 2009)

Multiplex QPCR but not ICTs are an essential adjunct to microscopy in the reference laboratory detection of malaria species specifically due to the superior LOD.

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Open Access | Methodology: Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group

December 14, 2009 - 11:30 -- Ingeborg van Schayk
Author(s): 
Samuel B Vezenegho, Chris Bass, Mirel Puinean, Martin S Williamson, Linda M Field, Maureen Coetzee, Lizette L Koekemoer
Reference: 
Malaria Journal 2009, 8:282 (9 December 2009)

In this study, three new high-throughput 'closed-tube' assays were developed and compared with the previously described AS-PCR technique.

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Open Access | Case report: Plasmodium malariae in Bangladesh

December 3, 2009 - 11:47 -- Patrick Sampao
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Author(s): 
W. Rahman, K. Chotivanich, K. Silamut, N. Tanomsing, A. Hossain, M.A. Faiz, A.M. Dondorp, R.J. Maude
Reference: 
Transactions of the Royal Society of Tropical Medicine and Hygiene, Volume 104, Issue 1, January 2010, Pages 78-80, doi:10.1016/j.trstmh.2009.06.014

We describe a 32-year-old Bangladeshi male presenting with severe malaria caused by a mono-infection with Plasmodium malariae. 

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