In this study, we evaluated the recently developed Partec rapid malaria test (PM) for the detection of Plasmodium spp. in human blood from patients in an area where malaria is endemic and compared the results with those of thick blood film Giemsa stain (GS) in terms of its performance and operational characteristics, using real-time (RT) PCR as the gold standard.
PCR-HRM is an inexpensive option for the determination of drug resistance profile in P. falciparum and will see increasing use as an alternative to sequencing and 5'nuclease PCR assays in reference laboratories or once PCR systems that are able to conduct HRM become commonplace.
The nested PCR method remains the method of choice for the detection of mixed malaria infections and especially of sub-clinical infections.
Our target was to determine the Plasmodium species in the southeast region of Turkey and the therapeutic efficacy of CQ used in the treatment of malaria.
During follow-up in antimalarial drug trials, treated subjects can be newly infected. PCR correction is used to distinguish this re-infection from drug failure (recrudescence) and to adjust final drug efficacy estimates.
We report a case of imported malaria in a boy, illustrating the epidemiological and clinical aspects of severe pediatric malaria.
Multiplex QPCR but not ICTs are an essential adjunct to microscopy in the reference laboratory detection of malaria species specifically due to the superior LOD.
In this study, three new high-throughput 'closed-tube' assays were developed and compared with the previously described AS-PCR technique.
We describe a 32-year-old Bangladeshi male presenting with severe malaria caused by a mono-infection with Plasmodium malariae.