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PCR

Summary of discordant results between rapid diagnosis tests, microscopy, and polymerase chain reaction for detecting Plasmodium mixed infection: a systematic review and meta-analysis

July 30, 2020 - 14:10 -- Open Access
Author(s): 
Kotepui M, Kotepui KU, De Jesus Milanez G, Masangkay FR
Reference: 
Sci Rep. 2020 Jul 29; 10(1):12765

Malaria rapid diagnostic tests (RDTs) are widely used to detect malaria parasites among patients who suspected malaria infections in malaria-endemic areas where microscopy is unavailable. Nevertheless, little is known about the performance of RDTs in detecting Plasmodium mixed infections. The present study aimed to evaluate the discordant results between RDTs and microscopy/polymerase chain reaction (PCR) in detecting Plasmodium mixed infections.

Systematic review and meta-analysis of diagnostic accuracy of Loop-mediated isothermal amplification (LAMP) methods compared to microscopy, PCR, and rapid diagnostic tests, for malaria diagnosis

July 14, 2020 - 15:50 -- Open Access
Author(s): 
Picot S, Cucherat M, Bienvenu AL
Reference: 
Int J Infect Dis. 2020 Jul 10:S1201-9712(20)30551-8

Diagnosis is a challenging issue in the way to malaria elimination. LAMP could be an alternative to conventional methods. Then, it is of interest to evaluate the diagnostic accuracy of LAMP for malaria compared to microscopy, PCR, and RDTs.

Therapeutic efficacy of artemether-lumefantrine in the treatment of uncomplicated Plasmodium falciparum malaria in Chewaka District, Ethiopia

July 14, 2020 - 09:17 -- Open Access
Author(s): 
Abdulhakim Abamecha, Daniel Yilma, Wondimagegn Addisu, Hassan El-Abid, Achim Ibenthal, Harald Noedl, Delenasaw Yewhalaw, Mohieddine Moumni and Alemseged Abdissa
Reference: 
Malaria Journal 2020 19:240, 10 July 2020

The efficacy of artemether-lumefantrine (AL) for treatment of uncomplicated Plasmodium falciparum malaria in south-western Ethiopia is poorly documented. Regular monitoring of drug efficacy is an important tool for supporting national treatment policies and practice. This study investigated the therapeutic efficacy of AL for the treatment of P. falciparum malaria in Ethiopia.

PCR correction strategies for malaria drug trials: updates and clarifications

January 20, 2020 - 15:01 -- Open Access
Author(s): 
Felger I, Snounou G, Hastings I, Moehrle JJ, Beck HP
Reference: 
Lancet Infect Dis. 2020 Jan; 20(1):e20-e25

Malaria drug trials conducted in endemic areas face a major challenge in their analysis because it is difficult to establish whether parasitaemia in blood samples collected after treatment indicate drug failure or a new infection acquired after treatment. It is therefore vital to reliably distinguish drug failures from new infections in order to obtain accurate estimates of drug failure rates.

Multiplex malaria antigen detection by bead-based assay and molecular confirmation by PCR shows no evidence of Pfhrp2 and Pfhrp3 deletion in Haiti

December 3, 2019 - 15:31 -- Open Access
Author(s): 
Camelia Herman, Curtis S. Huber, Sophie Jones, Laura Steinhardt, Mateusz M. Plucinski, Jean F. Lemoine, Michelle Chang, John W. Barnwell, Venkatachalam Udhayakumar and Eric Rogier
Reference: 
Malaria Journal 2019 18:380, 27 November 2019

The Plasmodium falciparum parasite is the only human malaria that produces the histidine-rich protein 2 and 3 (HRP2/3) antigens. Currently, HRP2/3 are widely used in malaria rapid diagnostic tests (RDTs), but several global reports have recently emerged showing genetic deletion of one or both of these antigens in parasites. Deletion of these antigens could pose a major concern for P. falciparum diagnosis in Haiti which currently uses RDTs based solely on the detection of the HRP2/3 antigens.

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