antibodies

NOT Open Access | Mice Pups Breastfed by Foster Mothers Whose Breast Milk Contains Plasmodium falciparum Recombinant SE36 Antigen Develop Specific Antibodies

Tags:

malaria, female mice, plasmodium falciparum, antibodies

Author(s):

Njoroge MM, Irungu Mwangi V, Owalla TJ, Ozwara H, Egwang TG

Reference:

Am J Trop Med Hyg. 2020 Dec 29

Intranasal instillation of SE36, a malaria vaccine candidate antigen, in lactating BALB/c female mice resulted in the appearance of the antigen in breast milk as demonstrated by sandwich ELISA and Western blot. Pups born of immunologically naive mice and breastfed on lactating foster mothers exposed intranasally to SE36 developed IgG anti-SE36 antibodies.

Evaluating the predictive performance of malaria antibodies and FCGR3B gene polymorphisms on Plasmodium falciparum infection outcome: a prospective cohort study

Tags:

malaria, antibodies, gene polymorphisms, plasmodium falciparum, children, Ghana

Author(s):

Duah Dwomoh, Bright Adu, Daniel Dodoo, Michael Theisen, Samuel Iddi and Thomas A. Gerds

Reference:

Malaria Journal 2020 19:307, 27 August 2020

Malaria antigen-specific antibodies and polymorphisms in host receptors involved in antibody functionality have been associated with different outcomes of Plasmodium falciparum infections. Thus, to identify key prospective malaria antigens for vaccine development, there is the need to evaluate the associations between malaria antibodies and antibody dependent host factors with more rigorous statistical methods. In this study, different statistical models were used to evaluate the predictive performance of malaria-specific antibodies and host gene polymorphisms on P. falciparum infection in a longitudinal cohort study involving Ghanaian children.

Vaccination with virosomally formulated recombinant CyRPA elicits protective antibodies against Plasmodium falciparum parasites in preclinical in vitro and in vivo models

Tags:

vaccination, CyRPA, antibodies, plasmodium falciparum, in vitro, in vivo

Author(s):

Marco Tamborrini, Julia Hauser, Anja Schäfer, Mario Amacker, Paola Favuzza, Kwak Kyungtak, Sylvain Fleury, Gerd Pluschke

Reference:

NPJ Vaccines. 2020; 5:9

The Plasmodium falciparum (Pf) cysteine-rich protective antigen (PfCyRPA) has emerged as a promising blood-stage candidate antigen for inclusion into a broadly cross-reactive malaria vaccine. This highly conserved protein among various geographical strains plays a key role in the red blood cell invasion process by P. falciparum merozoites, and antibodies against PfCyRPA can efficiently prevent the entry of the malaria parasites into red blood cells.

NOT Open Access | Improved stability of polyclonal antibodies: A case study with lyophilization-conserved antibodies raised against epitopes from the malaria parasite Plasmodium falciparum

Tags:

polyclonal, antibodies, lyophilization, malaria, parasite, plasmodium falciparum

Author(s):

Simon N, Sperber C, Voigtländer C, Born J, Gilbert DF, Seyferth S, Lee G, Kappes B, Friedrich O

Reference:

European Journal of Pharmaceutical Sciences, Volume 142, 15 January 2020, 105086

Antibodies can be produced as polyclonal (pAb) or monoclonal (mAb) liquid formulations with limited shelf-life. For pAbs, unlike mAbs, only little is known about excipients and lyophilization affecting antibody stability upon reconstitution. We used a model pAb directed against Plasmodium falciparum (Pf) pyridoxal 5′-phosphate synthase 2 (Pdx2) to systemically study effects of bulking agents (amino acids, phosphate buffers, salt solutions), sugar(alcohols), surfactants and protein additions (bovine serum albumin, BSA) in liquid pAb formulations (isolated or in combinations) on the activity to detect the antigen in Pf extracts by Western blots.

Functional Characterization and Comparison of Plasmodium falciparum Proteins as Targets of Transmission-blocking Antibodies

Tags:

Functional Characterization, Comparison, plasmodium falciparum, Proteins, transmission-blocking, antibodies

Author(s):

Nikolaeva D, Illingworth JJ, Biswas S, et al.

Reference:

Molecular & Cellular Proteomics January 1, 2020, 19 (1) 155-166

Plasmodium falciparum malaria continues to evade control efforts, utilizing highly specialized sexual-stages to transmit infection between the human host and mosquito vector. In a vaccination model, antibodies directed to sexual-stage antigens, when ingested in the mosquito blood meal, can inhibit parasite growth in the midgut and consequently arrest transmission. Despite multiple datasets for the Plasmodium sexual-stage transcriptome and proteome, there have been no rational screens to identify candidate antigens for transmission-blocking vaccine (TBV) development.

The persistence of naturally acquired antibodies and memory B cells specific to rhoptry proteins of Plasmodium vivax in patients from areas of low malaria transmission

Tags:

persistence, antibodies, memory B cells, rhoptry proteins, Plasmodium vivax, patients, malaria transmission

Author(s):

Kochayoo P, Changrob S, Wangriatisak K, Lee SK, Chootong P, Han ET

Reference:

Malaria Journal volume 18, 382 (2019)

Rhoptries are the large, paired, secretory organelles located at the apical tip of the malaria merozoite that are considered important for parasite invasion processes. Plasmodium vivax rhoptry proteins have been shown to induce humoral immunity during natural infections. Therefore, these proteins may be potential novel vaccine candidates. However, there is a lack of data on the duration of antibody and memory B cell (MBC) responses. Here, the longitudinal analysis of antibody and MBC responses to the P. vivax rhoptry proteins PvRALP1-Ecto and PvRhopH2 were monitored and analysed in individuals to determine their persistence.

Search form

You are here