The world's scientific and social network for malaria professionals
Subscribe to free Newsletter | 11033 malaria professionals are enjoying the free benefits of MalariaWorld today


Limitations of rapid diagnostic tests in malaria surveys in areas with varied transmission intensity in Uganda 2017-2019: Implications for selection and use of HRP2 RDTs

January 6, 2021 - 11:34 -- Open Access
Bosco AB, Nankabirwa JI, Kamya MR, et al.
PLoS One. 2020 Dec 31;15(12):e0244457

Plasmodium falciparum histidine-rich protein 2 (HRP2)-based rapid diagnostic tests (RDTs) are exclusively recommended for malaria diagnosis in Uganda; however, their functionality can be affected by parasite-related factors that have not been investigated in field settings.

NOT Open Access | An Antibody-Free Dual-Biomarker Rapid Enrichment Workflow (AnDREW) Improves the Sensitivity of Malaria Rapid Diagnostic Tests

November 19, 2020 - 13:37 -- NOT Open Access
Kantor AG, Markwalter CF, Nourani A, Wright DW
Anal Biochem. 2020 Nov 15:114020

Rapid diagnostic tests (RDTs) are critical to the success of malaria elimination campaigns. These tests are rapid, user-friendly, and field-deployable to resource-limited regions. However, RDTs demonstrate poor sensitivity because they can only tolerate a small (5 μL) volume of blood, which limits the amount of protein biomarker delivered to the test. We have developed the Antibody-free Dual-biomarker Rapid Enrichment Workflow (AnDREW) for purifying histidine-rich protein 2 (HRP2) and Plasmodium lactate dehydrogenase (PLDH) from large volume (150 μL) blood samples.

Clinical diagnostic evaluation of HRP2 and pLDH-based rapid diagnostic tests for malaria in an area receiving seasonal malaria chemoprevention in Niger

December 30, 2019 - 15:06 -- Open Access
Matthew E. Coldiron, Bachir Assao, Rebecca F. Grais, et al.
Malaria Journal 2019 18:443, 26 December 2019

Rapid diagnostic tests (RDT) for malaria are common, but their performance varies. Tests using histidine-rich protein 2 (HRP2) antigen are most common, and many have high sensitivity. HRP2 tests can remain positive for weeks after treatment, limiting their specificity and usefulness in high-transmission settings. Tests using Plasmodium lactate dehydrogenase (pLDH) have been less widely used but have higher specificity, mostly due to a much shorter time to become negative.

Monitoring the threatened utility of malaria rapid diagnostic tests by novel high-throughput detection of Plasmodium falciparum hrp2 and hrp3 deletions: A cross-sectional, diagnostic accuracy study

November 26, 2019 - 20:43 -- Open Access
Kreidenweiss A, Trauner F, Sandri TL, et al.
EBioMedicine. 2019 Nov 21. pii: S2352-3964(19)30723-6.

Plasmodium falciparum deficient for hrp2 and hrp3 genes are a threat to malaria management and elimination, since they escape widely used HRP2-based rapid diagnostic tests and treatment. Hrp2/hrp3 deletions are increasingly reported from all malaria endemic regions but are currently only identified by laborious methodologies.

Subscribe to RSS - hrp2