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The Effects of High-Altitude Windborne Migration on Survival, Oviposition, and Blood-Feeding of the African Malaria Mosquito, Anopheles gambiae s.l. (Diptera: Culicidae)

July 16, 2020 - 07:53 -- Open Access
Author(s): 
Sanogo ZL, Yaro AS, Dao A, Diallo M, Yossi O, Samaké D, Krajacich BJ, Faiman R, Lehmann T
Reference: 
J Med Entomol. 2020 Jul 15:tjaa137

Recent results of high-altitude windborne mosquito migration raised questions about the viability of these mosquitoes despite ample evidence that many insect species, including other dipterans, have been known to migrate regularly over tens or hundreds of kilometers on high-altitude winds and retain their viability. To address these concerns, we subjected wild Anopheles gambiae s.l.

NOT Open Access | piggyBac-mediated germline transformation of the malaria mosquito Anopheles sinensis (Diptera: Culicidae)

June 10, 2020 - 15:13 -- NOT Open Access
Author(s): 
Liu JG, Qiao L, Zhang JJ, Chen B, He ZB
Reference: 
Insect Sci. 2020 Jun 10

Anopheles sinensis is the most widely distributed species which mainly transmit the Plasmodium vivax malaria in China. Transgenic techniques have been successfully established in many other mosquitoes, but not previously reported in An. sinensis. In this study, the piggyBac transposable element vector pBac[3xP3‐EGFP afm] and the piggyBac helper plasmid phspBac were coinjected into preblastoderm eggs of An. sinensis, and the progenies were screened for eye EGFP fluorescence using a fluorescence microscope.

Assessing connectivity despite high diversity in island populations of a malaria mosquito

February 2, 2020 - 17:36 -- Open Access
Author(s): 
Christina M. Bergey, Martin Lukindu, Rachel M. Wiltshire, Michael C. Fontaine, Jonathan K. Kayondo, Nora J. Besansky
Reference: 
Evolutionary Applications, Volume13, Issue2, February 2020 Pages 417-431

Documenting isolation is notoriously difficult for species with vast polymorphic populations. High proportions of shared variation impede estimation of connectivity, even despite leveraging information from many genetic markers. We overcome these impediments by combining classical analysis of neutral variation with assays of the structure of selected variation, demonstrated using populations of the principal African malaria vector Anopheles gambiae. Accurate estimation of mosquito migration is crucial for efforts to combat malaria.

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