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human malaria parasite

Ubiquitin activation is essential for schizont maturation in Plasmodium falciparum blood-stage development

June 26, 2020 - 15:04 -- Open Access
Author(s): 
Green JL, Wu Y, Holder AA, et al.
Reference: 
PLoS Pathog. 2020 Jun 22; 16(6):e1008640

Ubiquitylation is a common post translational modification of eukaryotic proteins and in the human malaria parasite, Plasmodium falciparum (Pf) overall ubiquitylation increases in the transition from intracellular schizont to extracellular merozoite stages in the asexual blood stage cycle. Here, we identify specific ubiquitylation sites of protein substrates in three intraerythrocytic parasite stages and extracellular merozoites; a total of 1464 sites in 546 proteins were identified (data available via ProteomeXchange with identifier PXD014998). 469 ubiquitylated proteins were identified in merozoites compared with only 160 in the preceding intracellular schizont stage, suggesting a large increase in protein ubiquitylation associated with merozoite maturation.

Refining the transcriptome of the human malaria parasite Plasmodium falciparum using amplification-free RNA-seq

June 9, 2020 - 16:17 -- Open Access
Author(s): 
Chappell L, Ross P, Orchard L, Russell TJ, Otto TD, Berriman M, Rayner JC, Llinás M
Reference: 
BMC Genomics. 2020 Jun 8; 21(1):395

Plasmodium parasites undergo several major developmental transitions during their complex lifecycle, which are enabled by precisely ordered gene expression programs. Transcriptomes from the 48-h blood stages of the major human malaria parasite Plasmodium falciparum have been described using cDNA microarrays and RNA-seq, but these assays have not always performed well within non-coding regions, where the AT-content is often 90–95%.

A replication-competent late liver stage-attenuated human malaria parasite

June 3, 2020 - 07:07 -- Open Access
Author(s): 
Goswami D, Betz W, Kappe SH, et al.
Reference: 
JCI Insight. 2020 Jun 2:135589

Whole sporozoite vaccines engender sterilizing immunity against malaria in animal models and importantly, in humans. Gene editing allows for the removal of specific parasite genes, enabling generation of genetically attenuated parasite (GAP) strains for vaccination. Using rodent malaria parasites, we have previously shown that late liver stage-arresting replication-competent (LARC) GAPs confer superior protection when compared to early liver stage-arresting replication-deficient (EARD) GAPs and radiation-attenuated sporozoites.

Molecular dynamics simulations and biochemical characterization of Pf14-3-3 and PfCDPK1 interaction towards its role in growth of human malaria parasite

June 3, 2020 - 06:57 -- Open Access
Author(s): 
Jain R, Dey P, Gupta S, Pati S, Bhattacherjee A, Munde M, Singh S
Reference: 
Biochem J. 2020 Jun 2:BCJ20200145

Scaffold proteins play pivotal role as modulators of cellular processes by operating as multipurpose conformation clamps. 14-3-3 proteins are gold-standard scaffold modules that recognize phosphoSer/Thr (pS/pT) containing conserved motifs, and confer conformational changes leading to modulation of functional parameters of their target proteins. Modulation in functional activity of kinases has been attributed to their interaction with 14-3-3 proteins.

The male mosquito contribution towards malaria transmission: Mating influences the Anopheles female midgut transcriptome and increases female susceptibility to human malaria parasites

November 18, 2019 - 16:18 -- Open Access
Author(s): 
Farah Aida Dahalan, Thomas S. Churcher, Nikolai Windbichler, Mara K. N. Lawniczak
Reference: 
PLoS Pathog 15(11): e1008063

Mating causes dramatic changes in female physiology, behaviour, and immunity in many insects, inducing oogenesis, oviposition, and refractoriness to further mating. Females from the Anopheles gambiae species complex typically mate only once in their lifetime during which they receive sperm and seminal fluid proteins as well as a mating plug that contains the steroid hormone 20-hydroxyecdysone.

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