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in vivo

Structural and biophysical correlation of anti-NANP antibodies with in vivo protection against P. falciparum

February 25, 2021 - 10:12 -- Open Access
Author(s): 
Pholcharee T, Oyen D, Wilson IA, et al.
Reference: 
Nat Commun. 2021 Feb 16;12(1):1063

The most advanced P. falciparum circumsporozoite protein-based malaria vaccine, RTS,S/AS01 (RTS,S), confers partial protection but with antibody titers that wane relatively rapidly, highlighting the need to elicit more potent and durable antibody responses. Here, we elucidate crystal structures, binding affinities and kinetics, and in vivo protection of eight anti-NANP antibodies derived from an RTS,S phase 2a trial and encoded by three different heavy-chain germline genes.

Diatretol, an α, α'-dioxo-diketopiperazine, is a potent in vitro and in vivo antimalarial

January 19, 2021 - 16:04 -- Open Access
Author(s): 
Ishiyama A, Hokari R, Nonaka K, Chiba T, Miura H, Otoguro K, Iwatsuki M
Reference: 
J Antibiot (Tokyo). 2021 Jan 14:1-3

A fungal metabolite, diatretol, has shown to be a promising antimalarial agent.

Anti-Oxidant Potential and Antimalarial Effects of Acanthus polystachyus Delile (Acanthaceae) Against Plasmodium berghei: Evidence for in vivo Antimalarial Activity

December 23, 2020 - 10:32 -- Open Access
Author(s): 
Kifle ZD, Atnafie SA
Reference: 
J Exp Pharmacol. 2020 Dec 11;12:575-587

Malaria is among the most devastating and widespread tropical parasitic diseases which is more prevalent in developing countries. Acanthus polystachyus (Acanthaceae) leaves are traditionally used for the treatment of malaria in Ethiopia. This study aimed to investigate the in vivo antimalarial and in vitro antioxidant activity of the leaves extract of Acanthus polystachyus.

NOT Open Access | Plasmodium falciparum purine nucleoside phosphorylase as a model in the search for new inhibitors by high throughput screening

December 16, 2020 - 10:06 -- NOT Open Access
Author(s): 
Holanda RJ, Deves C, Pereira da Silva LH, et al.
Reference: 
Int J Biol Macromol. 2020 Dec 15;165(Pt B):1832-1841

Studies have shown that inhibition of Plasmodium falciparum Purine Nucleoside Phosphorylase (PfPNP) blocks the purine salvage pathway in vitro and in vivo. In this study, PfPNP was evaluated as a model in the search for new inhibitors using surface plasmon resonance (SPR). Its expression, purification, oligomeric state, kinetic constants, calorimetric parameters and kinetic mechanisms were obtained. PfPNP was immobilized on a CM5 sensor chip and sensorgrams were produced through binding the enzyme to the substrate MESG and interactions between molecules contained in 10 fractions of natural extracts.

NOT Open Access | Assessment of in vitro and in vivo antimalarial efficacy and GC-fingerprints of selected medicinal plant extracts

December 2, 2020 - 08:38 -- NOT Open Access
Author(s): 
Sachdeva C, Mohanakrishnan D, Kumar S, Kaushik NK
Reference: 
Exp Parasitol. 2020 Dec;219:108011

A hallmark of mortality and morbidity, malaria is affecting nearly half of the world's population. Emergence of drug-resistant strains of malarial parasite prompts identification and evaluation of medicinal plants and their constituents that may hold the key to a new and effective anti-malarial drug. In this context, nineteen methanolic extracts from seventeen medicinal plants were evaluated for anti-plasmodial potential against Plasmodium falciparum strain 3D7 (Chloroquine (CQ) sensitive) and INDO (CQ resistant) using fluorescence based SYBR-Green assay and for cytotoxic effects against mammalian cell lines.

Evaluation of in vivo anti-malarial potential of omidun obtained from fermented maize in Ibadan, Nigeria

November 24, 2020 - 13:42 -- Open Access
Author(s): 
Favour O. Omeiza, George O. Ademowo and Funmilola A. Ayeni
Reference: 
Malaria Journal 2020 19:414, 19 November 2020

The menace of resistance to anti-malarial drugs is a great challenge to malaria control, necessitating the search for new anti-malarial agents. This search has led to the exploration of natural products for efficacy in malaria therapy. Omidun is the supernatant of fermenting maize (ogi) slurry that has been widely investigated and reported to possess several health benefits and it is used traditionally as solvent for preparing anti-malarial herbs. However, there is no information on the anti-malarial activity of omidun itself. This study was conducted to investigate the prophylactic, curative and suppressive anti-malarial potential of omidun.

Plasmodium berghei K13 Mutations Mediate In Vivo Artemisinin Resistance That Is Reversed by Proteasome Inhibition

November 11, 2020 - 14:33 -- Open Access
Author(s): 
Simwela NV, Stokes BH, Aghabi D, Bogyo M, Fidock DA, Waters AP
Reference: 
mBio. 2020 Nov 10;11(6):e02312-20

The recent emergence of Plasmodium falciparum parasite resistance to the first line antimalarial drug artemisinin is of particular concern. Artemisinin resistance is primarily driven by mutations in the P. falciparum K13 protein, which enhance survival of early ring-stage parasites treated with the artemisinin active metabolite dihydroartemisinin in vitro and associate with delayed parasite clearance in vivo However, association of K13 mutations with in vivo artemisinin resistance has been problematic due to the absence of a tractable model.

NOT Open Access | Identification of 2,4-Disubstituted Imidazopyridines as Hemozoin Formation Inhibitors with Fast-Killing Kinetics and In Vivo Efficacy in the Plasmodium falciparum NSG Mouse Model

October 28, 2020 - 09:51 -- NOT Open Access
Author(s): 
Horatscheck A, Andrijevic A, Chibale K, et al.
Reference: 
J Med Chem. 2020 Oct 25

A series of 2,4-disubstituted imidazopyridines, originating from a SoftFocus Kinase library, was identified from a high throughput phenotypic screen against the human malaria parasite Plasmodium falciparum. Hit compounds showed moderate asexual blood stage activity.

From Circulation to Cultivation: Plasmodium In Vivo versus In Vitro

September 23, 2020 - 09:06 -- Open Access
Author(s): 
Brown AC, Guler JL
Reference: 
Trends Parasitol. 2020 Sep 18:S1471-4922(20)30224-5

Research on Plasmodium parasites has driven breakthroughs in reducing malaria morbidity and mortality. Experimental analysis of in vivo/ex vivo versus in vitro samples serve unique roles in Plasmodium research. However, these distinctly different environments lead to discordant biology between parasites in host circulation and those under laboratory cultivation.

Live In Vivo Imaging of Plasmodium Invasion of the Mosquito Midgut

September 5, 2020 - 14:44 -- Open Access
Author(s): 
Trisnadi N, Barillas-Mury C
Reference: 
mSphere. 2020 Sep 2;5(5):e00692-20

The mosquito midgut is a critical barrier that Plasmodium parasites must overcome to complete their developmental cycle and be transmitted to a new vertebrate host. Previous confocal studies with fixed infected midguts showed that ookinetes traverse midgut epithelial cells and cause irreversible tissue damage. Here, we investigated the spatiotemporal dynamics of ookinete midgut traversal and the response of midgut cells to invasion.

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