The world's scientific and social network for malaria professionals
Subscribe to free Newsletter | 10493 malaria professionals are enjoying the free benefits of MalariaWorld today

in vivo

In silico and in vivo anti-malarial investigation on 1-(heteroaryl)-2-((5-nitroheteroaryl)methylene) hydrazine derivatives

June 30, 2020 - 14:23 -- Open Access
Author(s): 
Azar Tahghighi, Seyed-Mahdi Mohamadi-Zarch, Hamzeh Rahimi, Mahya Marashiyan, Naseh Maleki-Ravasan and Ali Eslamifar
Reference: 
Malaria Journal 2020 19:231, 29 June 2020

Resistance of Plasmodium falciparum against common anti-malarial drugs emphasizes the need of alternative and more effective drugs. Synthetic derivatives of 1-(heteroaryl)-2-((5-nitroheteroaryl)methylene) hydrazine have showed in vitro anti-plasmodial activities. The present study aimed to evaluate the molecular binding and anti-plasmodial activity of synthetic compounds in vivo.

DFIQ, a Novel Quinoline Derivative, Shows Anticancer Potential by Inducing Apoptosis and Autophagy in NSCLC Cell and In Vivo Zebrafish Xenograft Models

June 1, 2020 - 16:20 -- Open Access
Author(s): 
Huang HW, Bow YD, Wang CY, Chen YC, Fu PR, Chang KF, Wang TW, Tseng CH, Chen YL, Chiu CC
Reference: 
Cancers (Basel). 2020 May 25; 12(5):E1348

Lung cancer is one of the deadliest cancers worldwide due to chemoresistance in patients with late-stage disease. Quinoline derivatives show biological activity against HIV, malaria, bacteriuria, and cancer. DFIQ is a novel synthetic quinoline derivative that induces cell death in both in vitro and in vivo zebrafish xenograft models. DFIQ induced cell death, including apoptosis, and the IC50 values were 4.16 and 2.31 μM at 24 and 48 h, respectively.

NOT Open Access | In silico, in vitro and in vivo evaluation of natural Bignoniaceous naphthoquinones in comparison with atovaquone targeting the selection of potential antimalarial candidates

June 1, 2020 - 16:06 -- NOT Open Access
Author(s): 
do Nascimento MFA, Borgati TF, de Souza LCR, Tagliati CA, de Oliveira AB
Reference: 
Toxicol Appl Pharmacol. 2020 May 25:115074

The natural naphthoquinones lapachol, α- and β-lapachone are found in Bignoniaceous Brazilian plant species of the Tabebuia genus (synonym Handroanthus) and are recognized for diverse bioactivities, including as antimalarial. The aim of the present work was to perform in silico, in vitro and in vivo studies to evaluating the antimalarial potential of these three naphthoquinones in comparison with atovaquone, a synthetic antimalarial.

Experimentally engineered mutations in a ubiquitin hydrolase, UBP-1, modulate in vivo susceptibility to artemisinin and chloroquine in Plasmodium berghei

April 29, 2020 - 09:05 -- Open Access
Author(s): 
Simwela NV, Hughes KR, Roberts AB, Rennie MT, Barrett MP, Waters AP
Reference: 
Antimicrob Agents Chemother. 2020 Apr 27. pii: AAC.02484-19

As resistance to artemisinins (current frontline drugs in malaria treatment) emerges in south East Asia, there is an urgent need to identify the genetic determinants and understand the molecular mechanisms underpinning such resistance. Such insights could lead to prospective interventions to contain resistance and prevent the eventual spread to other malaria endemic regions. Artemisinin reduced susceptibility in South East Asia (SEA) has been primarily linked to mutations in P. falciparum Kelch-13, which is currently widely recognised as a molecular marker of artemisinin resistance.

Development of an effective alternative model for in vivo hypnozoite-induced relapse infection: A Japanese macaque (Macaca fuscata) model experimentally infected with Plasmodium cynomolgi

March 3, 2020 - 15:09 -- Open Access
Author(s): 
Kawai S, Annoura T, Araki T, Shiogama Y, Soma S, Takano JI, Sato MO, Kaneko O, Yasutomi Y, Chigusa Y
Reference: 
Parasitology International, 2020 Feb 27:102096

In the present study, we demonstrate that the Japanese macaque (Macaca fuscata) can be used as an effective alternative in vivo model for investigating hypnozoite-induced relapsing infection caused by Plasmodium cynomolgi B strain, and that this model is comparable to the rhesus macaque model. Two female Japanese macaques (JM-1 and JM-2; aged 5 years; weighing about 4.0 kg) were used for the experiment.

NOT Open Access | Combination of zingerone and dihydroartemisinin presented synergistic antimalarial activity against Plasmodium berghei infection in BALB/c mice as in vivo model

March 2, 2020 - 14:07 -- NOT Open Access
Author(s): 
Ounjaijean S, Somsak V
Reference: 
Parasitology International, 20 February 2020, 102088

Malaria is a global health problem leading to the death of 435,000 cases in tropical and sub-tropical zones. Spread and emergence of increasing resistance to the antimalarial drugs are the major challenges in the control of malaria. Therefore, searching for alternative antimalarial drugs is urgently needed, and combination treatment preferred as an approach to address this. This study aimed to evaluate in vivo antimalarial activity of zingerone (ZN), and its combination with dihydroartemisinin (DHA) against Plasmodium berghei infected mice.

Vaccination with virosomally formulated recombinant CyRPA elicits protective antibodies against Plasmodium falciparum parasites in preclinical in vitro and in vivo models

February 11, 2020 - 16:23 -- Open Access
Author(s): 
Marco Tamborrini, Julia Hauser, Anja Schäfer, Mario Amacker, Paola Favuzza, Kwak Kyungtak, Sylvain Fleury, Gerd Pluschke
Reference: 
NPJ Vaccines. 2020; 5:9

The Plasmodium falciparum (Pf) cysteine-rich protective antigen (PfCyRPA) has emerged as a promising blood-stage candidate antigen for inclusion into a broadly cross-reactive malaria vaccine. This highly conserved protein among various geographical strains plays a key role in the red blood cell invasion process by P. falciparum merozoites, and antibodies against PfCyRPA can efficiently prevent the entry of the malaria parasites into red blood cells.

In Vivo Activity of Amodiaquine against Ebola Virus Infection

January 15, 2020 - 07:58 -- Open Access
Author(s): 
DeWald LE, Johnson JC, Gerhardt DM, Torzewski LM, Postnikova E, Honko AN, Janosko K, Huzella L, Dowling WE, Eakin AE, Osborn BL, Gahagen J, Tang L, Green CE, Mirsalis JC, Holbrook MR, Jahrling PB, Dyall J, Hensley LE
Reference: 
Scientific Reports, 2019 Dec 27; 9(1):20199

During the Ebola virus disease (EVD) epidemic in Western Africa (2013‒2016), antimalarial treatment was administered to EVD patients due to the high coexisting malaria burden in accordance with World Health Organization guidelines. In an Ebola treatment center in Liberia, EVD patients receiving the combination antimalarial artesunate-amodiaquine had a lower risk of death compared to those treated with artemether-lumefantrine.

Optimization of an in vivo model to study immunity to Plasmodium falciparum pre-erythrocytic stages

December 23, 2019 - 15:05 -- Open Access
Author(s): 
Yevel Flores-Garcia, Sonia M. Herrera, Hugo Jhun, Daniel W. Pérez-Ramos, C. Richter King, Emily Locke, Ramadevi Raghunandan and Fidel Zavala
Reference: 
Malaria Journal 2019 18:426, 18 December 2019

The circumsporozoite protein (CSP) of Plasmodium is a key surface antigen that induces antibodies and T-cells, conferring immune protection in animal models and humans. However, much of the work on CSP and immunity has been developed based on studies using rodent or non-human primate CSP antigens, which may not be entirely translatable to CSP expressed by human malaria parasites, especially considering the host specificity of the different species.

Mode of action of quinoline antimalarial drugs in red blood cells infected by Plasmodium falciparum revealed in vivo

November 18, 2019 - 16:15 -- Open Access
Author(s): 
Sergey Kapishnikov, Trine Staalsø, Yang Yang, Jiwoong Lee, Ana J. Pérez-Berná, Eva Pereiro, Yang Yang, Stephan Werner, Peter Guttmann, Leslie Leiserowitz, and Jens Als-Nielsen
Reference: 
PNAS November 12, 2019 116 (46) 22946-22952

The most widely used antimalarial drugs belong to the quinoline family. Their mode of action has not been characterized at the molecular level in vivo. We report the in vivo mode of action of a bromo analog of the drug chloroquine in rapidly frozen Plasmodium falciparum-infected red blood cells.

Medical Condition: 
Subscribe to RSS - in vivo