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gene amplification

Measurement of gene amplifications related to drug resistance in Plasmodium falciparum using droplet digital PCR

March 3, 2021 - 15:47 -- Open Access
Author(s): 
Suttipat Srisutham, Kanokon Suwannasin, Rungniran Sugaram, Arjen M. Dondorp and Mallika Imwong
Reference: 
Malaria Journal 2021 20:120, 28 February 2021

Copy number variations (CNVs) of the Plasmodium falciparum multidrug resistance 1 (pfmdr1), P. falciparum plasmepsin2 (pfplasmepsin2) and P. falciparum GTP cyclohydrolase 1 (pfgch1) genes are associated with anti-malarial drug resistance in P. falciparum malaria. Droplet digital PCR (ddPCR) assays have been developed for accurate assessment of CNVs in several human genes. The aim of the present study was to develop and validate ddPCR assays for detection of the CNVs of P. falciparum genes associated with resistance to anti-malarial drugs.

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