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DBS

Suitability of IgG responses to multiple Plasmodium falciparum antigens as markers of transmission intensity and pattern

April 28, 2021 - 14:33 -- Open Access
Author(s): 
Kyei-Baafour E, Oppong M, Ofori MF, et al.
Reference: 
PLoS One. 2021 Apr 22;16(4):e0249936

Detection of antibody reactivity to appropriate, specific parasite antigens may constitute a sensitive and cost-effective alternative to current tools to monitor malaria transmission across different endemicity settings. This study aimed to determine the suitability of IgG responses to a number of P. falciparum antigens as markers of transmission intensity and pattern.

Comparative performance of PCR using DNA extracted from dried blood spots and whole blood samples for malaria diagnosis: a meta-analysis

March 4, 2021 - 11:20 -- Open Access
Author(s): 
Mahittikorn A, Masangkay FR, Kotepui KU, De Jesus Milanez G, Kotepui M
Reference: 
Sci Rep. 2021 Mar 1;11(1):4845

Polymerase chain reaction (PCR) using deoxyribonucleic acid (DNA) extracted from dried blood spots (DBS) provides a fast, inexpensive, and convenient method for large-scale epidemiological studies. This study compared the performance of PCR between DNA extracted from DBS and DNA obtained from whole blood for detecting malarial parasites. Primary studies assessing the diagnostic performance of PCR using DNA extracted from DBS and whole blood for detecting malarial parasites were obtained from the ISI Web of Science, Scopus, and PubMed databases.

Optimization of whole-genome sequencing of Plasmodium falciparum from low-density dried blood spot samples

March 3, 2021 - 15:37 -- Open Access
Author(s): 
Noam B. Teyssier, Anna Chen, Elias M. Duarte, Rene Sit, Bryan Greenhouse and Sofonias K. Tessema
Reference: 
Malaria Journal 2021 20:116, 26 February 2021

Whole-genome sequencing (WGS) is becoming increasingly useful to study the biology, epidemiology, and ecology of malaria parasites. Despite ease of sampling, DNA extracted from dried blood spots (DBS) has a high ratio of human DNA compared to parasite DNA, which poses a challenge for downstream genetic analyses. The effects of multiple methods for DNA extraction, digestion of methylated DNA, and amplification were evaluated on the quality and fidelity of WGS data recovered from DBS.

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