The world's scientific and social network for malaria professionals
Subscribe to free Newsletter | 10739 malaria professionals are enjoying the free benefits of MalariaWorld today

auxin-inducible degron

NOT Open Access | Generation of Plasmodium yoelii malaria parasite for conditional degradation of proteins

December 16, 2020 - 12:24 -- NOT Open Access
Author(s): 
Liu C, Yang Z, Cai M, Shi Y, Cui H, Yuan J
Reference: 
Mol Biochem Parasitol. 2020 Dec 8:111346

The auxin-inducible degron (AID) system is a robust chemical-genetic method for manipulating endogenous protein level by conditional proteasomal degradation via a small molecule. So far, this system has not been adapted in the P. yoelii, an important and widely used Plasmodium rodent parasite model for malaria biology. Here, using the CRISPR/Cas9 genome editing method, we generated two marker-free transgenic P. yoelii parasite lines (eef1a-Tir1 and soap-Tir1) stably expressing the Oryza sativa gene tir1 under the promoters of eef1a and soap respectively.

Subscribe to RSS - auxin-inducible degron