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Validation of PfSNP-LAMP-Lateral Flow Dipstick for Detection of Single Nucleotide Polymorphism Associated with Pyrimethamine Resistance in Plasmodium falciparum

November 19, 2020 - 13:33 -- Open Access
Yongkiettrakul S, Kolié FR, Kongkasuriyachai D, Sattabongkot J, Nguitragool W, Nawattanapaibool N, Suansomjit C, Warit S, Kangwanrangsan N, Buates S
Diagnostics (Basel). 2020 Nov 13;10(11):E948

The loop-mediated isothermal amplification coupled with lateral flow dipstick (PfSNP-LAMP-LFD) was recently developed to detect single nucleotide polymorphism (AAT → ATT), corresponding to substitution of asparagine to isoleucine at amino acid position 51 in the P. falciparumdhfr-ts gene associated with antifolate resistance. In this present study, the PfSNP-LAMP-LFD was validated on 128 clinical malaria samples of broad ranged parasite densities (10 to 87,634 parasites per microliter of blood).

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