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qPCR

An Ultra-Sensitive Technique: Using Pv-mtCOX1 qPCR to Detect Early Recurrences of Plasmodium vivax in Patients in the Brazilian Amazon

January 5, 2021 - 15:31 -- Open Access
Author(s): 
Barbosa LRA, da Silva EL, Melo GC, et al.
Reference: 
Pathogens. 2020 Dec 30;10(1):E19

Early recurrence of Plasmodium vivax is a challenge for malaria control in the field, particularly because this species is associated with lower parasitemia, which hinders diagnosis and monitoring through blood smear testing. Early recurrences, defined as the persistence of parasites in the peripheral blood despite adequate drug dosages, may arise from resistance to chloroquine. The objective of the study was to estimate early recurrence of P. vivax in the Brazilian Amazon by using a highly-sensitive detection method, in this case, PCR.

Template copy number and the sensitivity of quantitative PCR for Plasmodium falciparum in asymptomatic individuals

August 19, 2020 - 09:32 -- Open Access
Author(s): 
Trevor A. Thompson, Mahamoudou B. Touré, Daouda Sanogo, Jeffrey G. Shaffer, Seydou O. Doumbia and Donald J. Krogstad
Reference: 
Malaria Journal 2020 19:295, 18 August 2020

The identification of asymptomatic individuals with Plasmodium falciparum infection is difficult because they do not seek medical treatment and often have too few asexual parasites detectable using microscopy or rapid diagnostic tests (≤ 200 parasites per μl). Quantitative PCR (qPCR) may provide greater sensitivity and permits estimation of the initial template DNA concentration. This study examined the hypothesis that qPCR assays using templates with higher copy numbers may be more sensitive for P. falciparum than assays based on templates with lower copy numbers.

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