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malachite-green loop mediated isothermal amplification (MG-LAMP)

Comparison of real time and malachite-green based loop-mediated isothermal amplification assays for the detection of Plasmodium vivax and P. falciparum

June 15, 2020 - 15:10 -- Open Access
Barazorda KA, Salas CJ, Bishop DK, Lucchi N, Valdivia HO
PLoS ONE 15(6): e0234263

The current context of malaria elimination requires urgent development and implementation of highly sensitive and specific methods for prompt detection and treatment of malaria parasites. Such methods should overcome current delays in diagnosis, allow the detection of low-density infections and address the difficulties in accessing remote endemic communities. In this study, we assessed the performance of the RealAmp and malachite-green loop mediated isothermal amplification (MG-LAMP) methodologies, using microscopy and conventional nested-PCR as reference techniques.

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