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anopheles sinensis (diptera: culicidae)

NOT Open Access | piggyBac-mediated germline transformation of the malaria mosquito Anopheles sinensis (Diptera: Culicidae)

June 10, 2020 - 15:13 -- NOT Open Access
Author(s): 
Liu JG, Qiao L, Zhang JJ, Chen B, He ZB
Reference: 
Insect Sci. 2020 Jun 10

Anopheles sinensis is the most widely distributed species which mainly transmit the Plasmodium vivax malaria in China. Transgenic techniques have been successfully established in many other mosquitoes, but not previously reported in An. sinensis. In this study, the piggyBac transposable element vector pBac[3xP3‐EGFP afm] and the piggyBac helper plasmid phspBac were coinjected into preblastoderm eggs of An. sinensis, and the progenies were screened for eye EGFP fluorescence using a fluorescence microscope.

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