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loop-mediated isothermal amplification

Diagnosis of Plasmodium vivax by Loop-Mediated Isothermal Amplification in Febrile Patient Samples from Loreto, Perú

August 5, 2020 - 16:06 -- Open Access
Nolasco O, Infante B, Contreras-Mancilla J, Incardona S, Ding XC, Gamboa D, Torres K.
Am J Trop Med Hyg. 2020 Aug 3

Plasmodium vivax is co-endemic with Plasmodium falciparum in Peru, and optimum management requires distinguishing these two species in the blood of patients. For the differential identification of P. vivax and other Plasmodium spp., the LoopampTM Malaria Pan Detection Kit in combination with the Loopamp Malaria Pv Detection Kit (Eiken Chemical Co. Ltd., Tokyo, Japan) was used to evaluate 559 whole blood samples collected in 2017 from febrile patients with suspected malaria attending different health facilities in the Loreto region.

Systematic review and meta-analysis of diagnostic accuracy of Loop-mediated isothermal amplification (LAMP) methods compared to microscopy, PCR, and rapid diagnostic tests, for malaria diagnosis

July 14, 2020 - 15:50 -- Open Access
Picot S, Cucherat M, Bienvenu AL
Int J Infect Dis. 2020 Jul 10:S1201-9712(20)30551-8

Diagnosis is a challenging issue in the way to malaria elimination. LAMP could be an alternative to conventional methods. Then, it is of interest to evaluate the diagnostic accuracy of LAMP for malaria compared to microscopy, PCR, and RDTs.

NOT Open Acces | Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria

April 6, 2020 - 14:50 -- NOT Open Access
Lai MY, Ooi CH, Jaimin JJ, Lau YL
Am J Trop Med Hyg. 2020 Mar 30

The incidence of zoonotic malaria, Plasmodium knowlesi, infection is increasing and now is the major cause of malaria in Malaysia. Here, we describe a WarmStart colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Plasmodium spp.

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