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human malaria

Positron emission tomography and magnetic resonance imaging in experimental human malaria to identify organ-specific changes in morphology and glucose metabolism: A prospective cohort study

June 1, 2021 - 15:43 -- Open Access
Woodford J, Gillman A, Jenvey P, Roberts J, Woolley S, Barber BE, Fernandez M, Rose S, Thomas P, Anstey NM, McCarthy JS
PLoS Med. 2021 May 26;18(5):e1003567

Plasmodium vivax has been proposed to infect and replicate in the human spleen and bone marrow. Compared to Plasmodium falciparum, which is known to undergo microvascular tissue sequestration, little is known about the behavior of P. vivax outside of the circulating compartment. This may be due in part to difficulties in studying parasite location and activity in life.

PfSPZ-CVac efficacy against malaria increases from 0% to 75% when administered in the absence of erythrocyte stage parasitemia: A randomized, placebo-controlled trial with controlled human malaria infection

June 1, 2021 - 12:11 -- Open Access
Murphy SC, Deye GA, Jackson LA, et al.
PLoS Pathog. 2021 May 28;17(5):e1009594

PfSPZ-CVac combines 'PfSPZ Challenge', which consists of infectious Plasmodium falciparum sporozoites (PfSPZ), with concurrent antimalarial chemoprophylaxis. In a previously-published PfSPZ-CVac study, three doses of 5.12x104 PfSPZ-CVac given 28 days apart had 100% vaccine efficacy (VE) against controlled human malaria infection (CHMI) 10 weeks after the last immunization, while the same dose given as three injections five days apart had 63% VE. Here, we conducted a dose escalation trial of similarly condensed schedules.

A nuclear protein, PfMORC confers melatonin dependent synchrony of the human malaria parasite P. falciparum in the asexual stage

January 26, 2021 - 15:12 -- Open Access
Singh MK, Tessarin-Almeida G, Dias BKM, Pereira PS, Costa F, Przyborski JM, Garcia CRS
Sci Rep. 2021 Jan 21;11(1):2057

The host hormone melatonin is known to modulate the asexual cell-cycle of the human malaria parasite Plasmodium falciparum and the kinase PfPK7 is fundamental in the downstream signaling pathways. The nuclear protein PfMORC displays a histidine kinase domain and is involved in parasite cell cycle control. By using a real-time assay, we show a 24 h (h) rhythmic expression of PfMORC at the parasite asexual cycle and the expression is dramatically changed when parasites were treated with 100 nM melatonin for 17 h.

Identification keys to the Anopheles mosquitoes of South America (Diptera: Culicidae). IV. Adult females

November 19, 2020 - 13:27 -- Open Access
Sallum MAM, Obando RG, Carrejo N, Wilkerson RC
Parasit Vectors. 2020 Nov 18;13(1):584

Morphological identification of adult females of described species of the genus Anopheles Meigen, 1818 in South America is problematic, but necessary due to their differing roles in the transmission of human malaria. The increase in the number of species complexes uncovered by molecular taxonomy challenges accurate identification using morphology. In addition, the majority of newly discovered species have not been formally described and in some cases the identities of the nominotypical species of species complexes have not been resolved. Here, we provide an up-to-date key to identify Neotropical Anopheles species using female external morphology and employing traditionally used and new characters.

NOT Open Access | Intrinsic fluorescence properties of antimalarial pyrido[1,2-a]benzimidazoles facilitate subcellular accumulation and mechanistic studies in the human malaria parasite Plasmodium falciparum

October 21, 2020 - 09:27 -- NOT Open Access
Korkor CM, Garnie LF, Amod L, Egan TJ, Chibale K
Org Biomol Chem. 2020 Oct 20

The intrinsic fluorescence properties of two related pyrido[1,2-a]benzimidazole antimalarial compounds suitable for the cellular imaging of the human malaria parasite Plasmodium falciparum without the need to attach extrinsic fluorophores are described. Although these compounds are structurally related, they have been shown by confocal microscopy to not only accumulate selectively within P. falciparum but to also accumulate differently in the organelles investigated.

Genetic diversity and neutral selection in Plasmodium vivax erythrocyte binding protein correlates with patient antigenicity

July 13, 2020 - 16:27 -- Open Access
Han JH, Cho JS, Han ET, et al.
PLoS Negl Trop Dis 14(7): e0008202

Plasmodium vivax is the most widespread and difficult to treat cause of human malaria. The development of vaccines against the blood stages of P. vivax remains a key objective for the control and elimination of vivax malaria. Erythrocyte binding-like (EBL) protein family members such as Duffy binding protein (PvDBP) are of critical importance to erythrocyte invasion and have been the major target for vivax malaria vaccine development.

Plasmodium falciparum translational machinery condones polyadenosine repeats

June 1, 2020 - 16:23 -- Open Access
Pavlovic Djuranovic S, Erath J, Andrews RJ, Bayguinov PO, Chung JJ, Chalker DL, Fitzpatrick JA, Moss WN, Szczesny P, Djuranovic S
Elife. 2020 May 29; 9:e57799

Plasmodium falciparum is causative agent of human malaria. Sixty percent of mRNAs from its extremely AT-rich (81%) genome harbor long polyadenosine (polyA) runs within their ORFs, distinguishing the parasite from its hosts and other sequenced organisms. Recent studies indicate polyA runs cause ribosome stalling and frameshifting, triggering mRNA surveillance pathways and attenuating protein synthesis.

A cutting-edge immunoinformatics approach for design of multi-epitope oral vaccine against dreadful human malaria

May 6, 2020 - 14:59 -- Open Access
Pritam M, Singh G, Swaroop S, Singh AK, Pandey B, Singh SP
Int J Biol Macromol. 2020 Apr 29. pii: S0141-8130(20)33050-6

Human malaria is a pathogenic disease mainly caused by Plasmodium falciparum, which was responsible for about 405,000 deaths globally in the year 2018. To date, several vaccine candidates have been evaluated for prevention, which failed to produce optimal output at various preclinical/clinical stages. This study is based on designing of polypeptide vaccines (PVs) against human malaria that cover almost all stages of life cycle of Plasmodium and for the same 5 genome derived predicted antigenic proteins (GDPAP) have been used.

Not Open Access | Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections

March 3, 2020 - 12:38 -- NOT Open Access
Lalremruata A, Nguyen TT, Mordmüller B, et al.
J Clin Microbiol. 2020 Feb 26. pii: JCM.01879-19

Microscopy and rapid diagnostic tests (RDTs) are the main diagnostic tools for malaria but fail to detect low-density parasitemia that are important for maintaining malaria transmission. To complement existing diagnostic methods, an isothermal reverse transcription recombinase polymerase amplification and lateral flow assay (RT-RPA) was developed.

Fast and fierce versus slow and smooth: Heterogeneity in immune responses to Plasmodium in the controlled human malaria infection model

January 20, 2020 - 14:39 -- Open Access
Yap XZ, McCall MBB, Sauerwein RW
Immunological Reviews, Volume293, Issue1, January 2020

Controlled human malaria infection (CHMI) is an established model in clinical malaria research. Upon exposure to Plasmodium falciparum parasites, malaria‐naive volunteers differ in dynamics and composition of their immune profiles and subsequent capacity to generate protective immunity. CHMI volunteers are either inflammatory responders who have prominent cellular IFN‐γ production primarily driven by adaptive T cells, or tempered responders who skew toward antibody‐mediated humoral immunity. When exposed to consecutive CHMIs under antimalarial chemoprophylaxis, individuals who can control parasitemia after a single immunization (fast responders) are more likely to be protected against a subsequent challenge infection.


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