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gene drive

Next-generation gene drive for population modification of the malaria vector mosquito, Anopheles gambiae

August 26, 2020 - 14:56 -- Open Access
Carballar-Lejarazú R, Ogaugwu C, Tushar T, Kelsey A, Pham TB, Murphy J, Schmidt H, Lee Y, Lanzaro GC, James AA
Proc Natl Acad Sci U S A. 2020 Aug 24:202010214

A Cas9/guide RNA-based gene drive strain, AgNosCd-1, was developed to deliver antiparasite effector molecules to the malaria vector mosquito, Anopheles gambiae The drive system targets the cardinal gene ortholog producing a red-eye phenotype. Drive can achieve 98 to 100% in both sexes and full introduction was observed in small cage trials within 6 to 10 generations following a single release of gene-drive males.

Modelling the suppression of a malaria vector using a CRISPR-Cas9 gene drive to reduce female fertility

August 13, 2020 - 11:38 -- Open Access
North AR, Burt A, Godfray HCJ
BMC Biol. 2020 Aug 11;18(1):98

Gene drives based on CRISPR-Cas9 technology are increasingly being considered as tools for reducing the capacity of mosquito populations to transmit malaria, and one of the most promising options is driving endonuclease genes that reduce the fertility of female mosquitoes. In particular, there is much interest in constructs that target the conserved mosquito doublesex (dsx) gene such that the emergence of functional drive-resistant alleles is unlikely. Proof of principle that these constructs can lead to substantial population suppression has been obtained in population cages, and they are being evaluated for use in sub-Saharan Africa. Here, we use simulation modelling to understand the factors affecting the spread of this type of gene drive over a one million-square kilometre area of West Africa containing substantial environmental and social heterogeneity.

Toward the Definition of Efficacy and Safety Criteria for Advancing Gene Drive-Modified Mosquitoes to Field Testing

March 17, 2020 - 15:16 -- Open Access
James SL, Marshall JM, Christophides GK, Okumu FO, Nolan T
Vector Borne Zoonotic Dis. 2020 Mar 9

Mosquitoes containing gene drive systems are being developed as complementary tools to prevent transmission of malaria and other mosquito-borne diseases. As with any new tool, decision makers and other stakeholders will need to balance risks (safety) and benefits (efficacy) when considering the rationale for testing and deploying gene drive-modified mosquito products. Developers will benefit from standards for judging whether an investigational gene drive product meets acceptability criteria for advancing to field trials.

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