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qRT-PCR

Sources of persistent malaria transmission in a setting with effective malaria control in eastern Uganda: a longitudinal, observational cohort study

June 22, 2021 - 15:39 -- Open Access
Author(s): 
Andolina C, Rek JC, Bousema T, et al.
Reference: 
Lancet Infect Dis. 2021 Jun 16:S1473-3099(21)00072-4

Symptomatic malaria cases reflect only a small proportion of all Plasmodium spp infections. Many infected individuals are asymptomatic, and persistent asymptomatic Plasmodium falciparum infections are common in endemic settings. We aimed to quantify the contribution of symptomatic and asymptomatic infections to P falciparum transmission in Tororo, Uganda.

NOT Open Access | Anopheles dirus yellow-g mediates Plasmodium vivax infection

June 9, 2021 - 07:22 -- NOT Open Access
Author(s): 
Mongkol W, Pomun T, Nguitragool W, Kumpitak C, Duangmanee A, Sattabongkot J, Kubera A
Reference: 
Trop Med Int Health. 2021 Jun 5

Our previous transcriptome analysis of Anopheles dirus revealed up regulation of the An. dirus yellow-g gene upon ingestion of Plasmodium vivax-infected blood. This gene belongs to the yellow gene family, but its role regarding P. vivax infection is not known and remains to be validated. The aim of this study was to investigate the role of the An. dirus yellow-g gene in P. vivax infection.

Assays for quantification of male and female gametocytes in human blood by qRT-PCR in the absence of pure sex-specific gametocyte standards

June 26, 2020 - 10:45 -- Open Access
Author(s): 
Claire Y. T. Wang, Emma Ballard, Stacey Llewellyn, Louise Marquart, Teun Bousema, James S. McCarthy and Katharine A. Collins
Reference: 
Malaria Journal 2020 19:218, 23 June 2020

Malaria transmission from humans to Anopheles mosquitoes requires the presence of gametocytes in human peripheral circulation, and the dynamics of transmission are determined largely by the density and sex ratio of the gametocytes. Molecular methods are thus employed to measure gametocyte densities, particularly when assessing transmission epidemiology and the efficacy of transmission-blocking interventions. However, accurate quantification of male and female gametocytes with molecular methods requires pure male and female gametocytes as reference standards, which are not widely available.

qRT-PCR versus IFA-based Quantification of Male and Female Gametocytes in Low-Density Plasmodium falciparum Infections and Their Relevance for Transmission

February 14, 2020 - 16:38 -- Open Access
Author(s): 
Gruenberg M, Hofmann NE, Nate E, Karl S, Robinson LJ, Lanke K, Smith TA, Bousema T, Felger I
Reference: 
J Infect Dis. 2020 Feb 3;221(4):598-607

Accurate quantification of female and male gametocytes and sex ratios in asymptomatic low-density malaria infections are important for assessing their transmission potential. Gametocytes often escape detection even by molecular methods, therefore ultralow gametocyte densities were quantified in large blood volumes.

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