The world's scientific and social network for malaria professionals
Subscribe to free Newsletter | 10366 malaria professionals are enjoying the free benefits of MalariaWorld today

lyophilization

NOT Open Access | Improved stability of polyclonal antibodies: A case study with lyophilization-conserved antibodies raised against epitopes from the malaria parasite Plasmodium falciparum

January 20, 2020 - 16:16 -- NOT Open Access
Author(s): 
Simon N, Sperber C, Voigtländer C, Born J, Gilbert DF, Seyferth S, Lee G, Kappes B, Friedrich O
Reference: 
European Journal of Pharmaceutical Sciences, Volume 142, 15 January 2020, 105086

Antibodies can be produced as polyclonal (pAb) or monoclonal (mAb) liquid formulations with limited shelf-life. For pAbs, unlike mAbs, only little is known about excipients and lyophilization affecting antibody stability upon reconstitution. We used a model pAb directed against Plasmodium falciparum (Pf) pyridoxal 5′-phosphate synthase 2 (Pdx2) to systemically study effects of bulking agents (amino acids, phosphate buffers, salt solutions), sugar(alcohols), surfactants and protein additions (bovine serum albumin, BSA) in liquid pAb formulations (isolated or in combinations) on the activity to detect the antigen in Pf extracts by Western blots.

Subscribe to RSS - lyophilization