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red blood cell

Red blood cell blood group A antigen level affects the ability of heparin and PfEMP1 antibodies to disrupt Plasmodium falciparum rosettes

November 23, 2021 - 09:29 -- Open Access
Pontus Hedberg, Madle Sirel, Kirsten Moll, Mpungu Steven Kiwuwa, Petter Höglund, Ulf Ribacke and Mats Wahlgren
Malaria Journal 2021 20:441, 18 November 2021

The histo-blood group ABO system has been associated with adverse outcomes in COVID-19, thromboembolic diseases and Plasmodium falciparum malaria. An integral part of the severe malaria pathogenesis is rosetting, the adherence of parasite infected red blood cells (RBCs) to uninfected RBCs. Rosetting is influenced by the host’s ABO blood group (Bg) and rosettes formed in BgA have previously been shown to be more resilient to disruption by heparin and shield the parasite derived surface antigens from antibodies. However, data on rosetting in weak BgA subgroups is scarce and based on investigations of relatively few donors.

NOT Open Access | Lipopolysaccharide preconditioning augments phagocytosis of malaria-parasitized red blood cells by bone marrow-derived macrophages in the liver, thereby increasing the murine survival after Plasmodium yoelii infection

August 25, 2021 - 15:49 -- NOT Open Access
Ono T, Yamaguchi Y, Nakashima H, Nakashima M, Ishikiriyama T, Seki S, Kinoshita M
Infect Immun. 2021 Aug 23:IAI0024921

Malaria remains a grave concern for humans, as effective medical countermeasures for Plasmodium infection have yet to be developed. Phagocytic clearance of parasitized red blood cells (pRBCs) by macrophages is an important front-line innate host defense against Plasmodium infection. We previously showed that repeated injections of low-dose lipopolysaccharide (LPS) prior to bacterial infection, called LPS preconditioning, strongly augmented phagocytic/bactericidal activity in murine macrophages. However, if LPS preconditioning prevents murine Plasmodium infection is unclear.

Not Open Access | Modeling the effects of malaria on red blood cell binding at the vascular interface

August 25, 2021 - 15:37 -- NOT Open Access
Clifton LA
Biophys J. 2021 Aug 17;120(16):3240-3241

Malaria is one of the largest diseases affecting tropical and subtropical regions, with more than 200 million cases and between 400,000 and 500,000 deaths annually, disproportionately afflicting the young, elderly, or immune compromised (1, 2). Infamously spread by infected mosquitoes, the disease is caused by several parasitic species of Plasmodium (P. falciparum, P. malariae, P. vivax, P. ovale, and P. knowlesi).

Genetic polymorphism of the extracellular region in surface associated interspersed 1.1 gene of Plasmodium falciparum field isolates from Thailand

August 18, 2021 - 16:27 -- Open Access
Natpasit Chaianantakul, Tippawan Sungkapong, Jirapinya Changpad, Keawalin Thongma, Sasiwimon Sim-ut and Morakot Kaewthamasorn
Malaria Journal 2021 20:343, 16 August 2021

A novel variable surface antigens (VSAs), Surface-associated interspersed proteins (SUFRINs), is a protein that is modified on the surface of infected red blood cell (iRBC). Modified proteins on the iRBC surface cause severe malaria, which can lead to death throughout the life cycle of a malaria parasite. Previous study suggested that SURFIN1.1 is an immunogenic membrane-associated protein which was encoded by using the surf1.1 gene expressed during the trophozoite and schizont stages. This study aimed to identify the regions of SURFIN1.1 and investigate the genetic diversity of the extracellular region of the surf1.1 gene.

NOT Open Access | Structured to conquer: transport across the Plasmodium parasitophorous vacuole

August 11, 2021 - 14:36 -- NOT Open Access
Garten M, Beck JR
Curr Opin Microbiol. 2021 Aug 7;63:181-188

During the vertebrate stage of the Plasmodium life cycle, obligate intracellular malaria parasites establish a vacuolar niche for replication, first within host hepatocytes at the pre-patent liver-stage and subsequently in erythrocytes during the pathogenic blood-stage. Survival in this protective microenvironment requires diverse transport mechanisms that enable the parasite to transcend the vacuolar barrier.

Not Open Access | Vivax malaria detection using a parasitic red blood cell flag generated by the Sysmex XN-9000 hematology analyzer

August 11, 2021 - 14:30 -- NOT Open Access
Huh HJ, Chung JW, Park SY, Chae SL
Int J Lab Hematol. 2021 Aug 9

A Sysmex XN-series hematology analyzer (Sysmex), the next generation up from the Sysmex XE-series, can provide information regarding malaria infection in the form of a parasitic red blood cell (pRBC) flag. This study aimed to determine the usefulness of the pRBC flag for early detection and follow-up in patients infected with Plasmodium vivax.

NOT Open Access | An in vitro study on the differentiated metabolic mechanism of chloroquine-resistant Plasmodium falciparum using high-resolution metabolomics

August 4, 2021 - 12:20 -- NOT Open Access
Na J, Zhang J, Choe YL, Lim CS, Park YH
J Toxicol Environ Health A. 2021 Aug 1:1-16

Chloroquine (CQ) is an important drug used therapeutically for treatment of malaria. However, due to limited number of studies on metabolic targets of chloroquine (CQ), it is difficult to attribute mechanisms underlying resistance associated with usage of this drug. The present study aimed to investigate the metabolic signatures of CQ-resistant Plasmodium falciparum (PfDd2) compared to CQ-sensitive Plasmodium falciparum (Pf3D7).

Evolution of transcriptional control of antigenic variation and virulence in human and ape malaria parasites

July 14, 2021 - 10:02 -- Open Access
Gross MR, Hsu R, Deitsch KW
BMC Ecol Evol. 2021 Jul 8;21(1):139

The most severe form of human malaria is caused by the protozoan parasite Plasmodium falciparum. This unicellular organism is a member of a subgenus of Plasmodium called the Laverania that infects apes, with P. falciparum being the only member that infects humans. The exceptional virulence of this species to humans can be largely attributed to a family of variant surface antigens placed by the parasites onto the surface of infected red blood cells that mediate adherence to the vascular endothelium. These proteins are encoded by a large, multicopy gene family called var, with each var gene encoding a different form of the protein. By changing which var gene is expressed, parasites avoid immune recognition, a process called antigenic variation that underlies the chronic nature of malaria infections.

Inter-study and time-dependent variability of metabolite abundance in cultured red blood cells

July 7, 2021 - 14:42 -- Open Access
Shivendra G. Tewari, Krithika Rajaram, Russell P. Swift, Bobby Kwan, Jaques Reifman, Sean T. Prigge and Anders Wallqvist
Malaria Journal 2021 20:299, 2 July 2021

Cultured human red blood cells (RBCs) provide a powerful ex vivo assay platform to study blood-stage malaria infection and propagation. In recent years, high-resolution metabolomic methods have quantified hundreds of metabolites from parasite-infected RBC cultures under a variety of perturbations. In this context, the corresponding control samples of the uninfected culture systems can also be used to examine the effects of these perturbations on RBC metabolism itself and their dependence on blood donors (inter-study variations).

The Plasmodium falciparum Rh5 invasion protein complex reveals an excess of rare variant mutations

June 23, 2021 - 14:39 -- Open Access
Leonard Ndwiga, Victor Osoti, Kevin Omondi Ochwedo, Kevin Wamae, Philip Bejon, Julian C. Rayner, George Githinji and Lynette Isabella Ochola-Oyier
Malaria Journal 2021 20:278, 23 June 2021

The invasion of the red blood cells by Plasmodium falciparum merozoites involves the interplay of several proteins that are also targets for vaccine development. The proteins PfRh5-PfRipr-PfCyRPA-Pfp113 assemble into a complex at the apical end of the merozoite and are together essential for erythrocyte invasion. They have also been shown to induce neutralizing antibodies and appear to be less polymorphic than other invasion-associated proteins, making them high priority blood-stage vaccine candidates. Using available whole genome sequencing data (WGS) and new capillary sequencing data (CS), this study describes the genetic polymorphism in the Rh5 complex in P. falciparum isolates obtained from Kilifi, Kenya.


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