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guanidine hydrochloride

Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride

April 29, 2019 - 13:17 -- Open Access
Author(s): 
Sutharinee Ngernna, Anongruk Chim-ong, Wanlapa Roobsoong, Jetsumon Sattabongkot, Liwang Cui and Wang Nguitragool
Reference: 
Malaria Journal 2019 18:148, 25 April 2019

Long-term in vitro culture of blood stage Plasmodium parasites invariably leads to asynchronous parasite development. The most often used technique to synchronize Plasmodium falciparum culture is sorbitol treatment, which differentially induces osmotic lysis of trophozoite- and schizont-infected red blood cells due to presence of the new permeation pathways in the membranes of these cells. However, sorbitol treatment does not work well when used to synchronize the culture-adapted Plasmodium knowlesi A1-H.1 line.

Medical Treatment: 
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