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NOT Open Access | An Antibody-Free Dual-Biomarker Rapid Enrichment Workflow (AnDREW) Improves the Sensitivity of Malaria Rapid Diagnostic Tests

November 19, 2020 - 13:37 -- NOT Open Access
Kantor AG, Markwalter CF, Nourani A, Wright DW
Anal Biochem. 2020 Nov 15:114020

Rapid diagnostic tests (RDTs) are critical to the success of malaria elimination campaigns. These tests are rapid, user-friendly, and field-deployable to resource-limited regions. However, RDTs demonstrate poor sensitivity because they can only tolerate a small (5 μL) volume of blood, which limits the amount of protein biomarker delivered to the test. We have developed the Antibody-free Dual-biomarker Rapid Enrichment Workflow (AnDREW) for purifying histidine-rich protein 2 (HRP2) and Plasmodium lactate dehydrogenase (PLDH) from large volume (150 μL) blood samples.

Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma

January 14, 2020 - 09:27 -- Open Access
Xavier Martiáñez-Vendrell, Alfons Jiménez, Alfredo Mayor, et al.
Malaria Journal 2020 19:12, 9 January 2020

Malaria diagnostics by rapid diagnostic test (RDT) relies primarily on the qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and Plasmodium spp lactate dehydrogenase (pLDH). As novel RDTs with increased sensitivity are being developed and implemented as point of care diagnostics, highly sensitive laboratory-based assays are needed for evaluating RDT performance. Here, a quantitative suspension array technology (qSAT) was developed, validated and applied for the simultaneous detection of PfHRP2 and pLDH in a variety of biological samples (whole blood, plasma and dried blood spots) from individuals living in different endemic countries.

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