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genetic manipulation

Expansion, in vivo-ex vivo cycling, and genetic manipulation of primary human hepatocytes

January 14, 2020 - 10:13 -- Open Access
Michailidis E, Vercauteren K, de Jong YP, et al.
Proc Natl Acad Sci U S A. 2020 Jan 8. pii: 201919035

Primary human hepatocytes (PHHs) are an essential tool for modeling drug metabolism and liver disease. However, variable plating efficiencies, short lifespan in culture, and resistance to genetic manipulation have limited their use. Here, we show that the pyrrolizidine alkaloid retrorsine improves PHH repopulation of chimeric mice on average 10-fold and rescues the ability of even poorly plateable donor hepatocytes to provide cells for subsequent ex vivo cultures.

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