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phenotypes

NOT Open Access | A modified two-color flow cytometry assay to quantify in-vitro reinvasion and determine invasion phenotypes at low Plasmodium falciparum parasitemia

November 4, 2020 - 16:03 -- NOT Open Access
Author(s): 
Ngoh IA, Anong DN, Fru JC, Bojang F, Mbye H, Amambua-Ngwa A
Reference: 
Exp Parasitol. 2020 Nov;218:107969

Invasion of human red blood cells (RBCs) by Plasmodium parasites is a crucial yet poorly characterised phenotype. Two-color flow cytometry (2cFCM) promises to be a very sensitive and high throughput method for phenotyping parasite invasion. However, current protocols require high (~1.0%) parasitemia for assay set-up and need to be adapted for low parasitemia samples, which are becoming increasingly common in low transmission settings.

Prevalence of CYP2D6 Genotypes and Predicted Phenotypes in a Cohort of Cambodians at High Risk for Infections with Plasmodium vivax

August 12, 2020 - 15:12 -- Open Access
Author(s): 
Spring MD, Lon C, Sok S, Sea D, Wojnarski M, Chann S, Kuntawunginn W, Kheang Heng T, Nou S, Arsanok M, Sriwichai S, Vanachayangkul P, Lin JT, Manning JE, Jongsakul K, Pichyangkul S, Satharath P, Smith PL, Dysoley L, Saunders DL, Waters NC
Reference: 
Am J Trop Med Hyg. 2020 Aug;103(2):756-759.

Clinical failure of primaquine (PQ) has been demonstrated in people with CYP450 2D6 genetic polymorphisms that result in reduced or no enzyme activity. The distribution of CYP2D6 genotypes and predicted phenotypes in the Cambodian population is not well described. Surveys in other Asian countries have shown an approximate 50% prevalence of the reduced activity CYP2D6 allele *10, which could translate into increased risk of PQ radical cure failure and repeated relapses, making interruption of transmission and malaria elimination difficult to achieve.

Genotypes and phenotypes of resistance in Ecuadorian Plasmodium falciparum

December 17, 2019 - 15:56 -- Open Access
Author(s): 
Gabriela Valenzuela, L. Enrique Castro, Julio Valencia-Zamora, Claudia A. Vera-Arias, Petra Rohrbach and Fabián E. Sáenz
Reference: 
Malaria Journal 2019 18:415, 10 December 2019

Malaria continues to be endemic in the coast and Amazon regions of Ecuador. Clarifying current Plasmodium falciparum resistance in the country will support malaria elimination efforts. In this study, Ecuadorian P. falciparum parasites were analysed to determine their drug resistance genotypes and phenotypes.

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