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histidine

Not Open Access | Enhanced purification and characterization of the PfeIF4A (PfH45) helicase from Plasmodium Falciparum using a codon-optimised clone

August 29, 2012 - 15:02 -- Patrick Sampao
Author(s): 
Luke Evans, Darren Gowers, Keith Firman, James Youell
Reference: 
Protein Expression and Purification, Volume 85, Issue 1, September 2012, Pages 1-8

MalariaWorldWith the intention of investigating the DNA strand displacement properties of Plasmodium falciparum helicase PfeIF4A (formerly known as PfH45) a codon-optimized gene for expression in Escherichia coli has been produced. Several histidine-containing proteins with intrinsic helicase activity were captured from the bacterial sonicate by initial Ni2+-chromatography.

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Not Open Access | Identification of optimal epitopes for Plasmodium falciparum rapid diagnostic tests that target histidine-rich proteins 2 and 3.

July 18, 2012 - 15:25 -- Patrick Sampao
Author(s): 
Lee N, Gatton ML, Pelecanos A, Bubb M, Gonzalez I, Bell D, Cheng Q, McCarthy JS.
Reference: 
J Clin Microbiol. 2012 Apr;50(4):1397-405

This study therefore informs the selection of MAbs to include in malaria RDTs as well as in the generation of improved MAbs that should improve the performance of HRP-detecting malaria RDTs.

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Monoclonal Antibodies Against Recombinant Histidine-Rich Protein 2 of Plasmodium falciparum and Their Use in Malaria Diagnosis

January 19, 2012 - 15:04 -- Patrick Sampao
Author(s): 
Samuel Merwyn, Natarajan Gopalan, Anil Kumar Singh, Ganga Prasad Rai, and Gauri Shanker Agarwal.
Reference: 
Hybridoma. December 2011, 30(6): 519-524.

MalariaWorldIn this study, a set of five monoclonal antibodies against recombinant HRPII (rHRPII) were generated and assessed for their potential in diagnostics.

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