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Overexpression, purification and assessment of cyclosporin binding of a family of cyclophilins and cyclophilin-like proteins of the human malarial parasite Plasmodium falciparum

June 13, 2011 - 14:14 -- Patrick Sampao
Alejandro Marín-Menéndez, Angus Bell
Protein Expression and Purification, Volume 78, Issue 2, August 2011, Pages 225-234

Previously a pET vector conferring a C-terminal His6 tag was used for recombinant expression and purification of one member of the P. falciparum cyclophilin family in Escherichia coli. The approach here was to use an identical method to produce all of the other members of this family and thereby allow the most consistent functional comparisons.

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Open Access | Oral Presentation: Identification and characterization of novel Plasmodium falciparum cyclophilins and their roles in the antimalarial actions of cyclosporin A and derivatives

November 4, 2010 - 07:38 -- Patrick Sampao
Alejandro Marin-Menendez, Angus Bell
Malaria Journal 2010, 9(Suppl 2):O3 (20 October 2010)

We are interested in the role (if any) played by cyclophilins in parasite killing by cyclosporins.

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