Previously a pET vector conferring a C-terminal His6 tag was used for recombinant expression and purification of one member of the P. falciparum cyclophilin family in Escherichia coli. The approach here was to use an identical method to produce all of the other members of this family and thereby allow the most consistent functional comparisons.
Protein Expression and Purification, Volume 78, Issue 2, August 2011, Pages 225-234
Malaria Journal 2010, 9(Suppl 2):O3 (20 October 2010)
We are interested in the role (if any) played by cyclophilins in parasite killing by cyclosporins.