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PCR-Based

Parasitology: PCR-Based Pooling of Dried Blood Spots for Detection of Malaria Parasites: Optimization and Application to a Cohort of Ugandan Children

October 1, 2010 - 08:57 -- Patrick Sampao
Author(s): 
Michelle S. Hsiang, Michael Lin, Christian Dokomajilar, Jordan Kemere, Christopher D. Pilcher, Grant Dorsey, and Bryan Greenhouse
Reference: 
J. Clin. Microbiol., Oct 2010; 48: 3539 - 3543.

We first used laboratory-prepared samples to compare 2 DNA extraction and 4 PCR detection methods across a range of pool sizes and parasite densities. Pooled Chelex extraction of DNA, followed by nested PCR of cytochrome b, was the optimal strategy, allowing reliable detection of a single low-parasitemic sample (100 parasites/µl) in pool sizes up to 50.

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