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diagnosis

Short Reports: Tales from the bench: laboratory diagnosis of malaria

June 7, 2011 - 14:06 -- Patrick Sampao
Author(s): 
Hilary Edwards MSc MA
Reference: 
Trop Doct 2011;41:106-107

This is a practical laboratory overview of current detection methods for malaria parasites, suitable for use in developing countries.

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Novel Nested Direct PCR Technique for Malaria Diagnosis Using Filter Paper Samples

April 5, 2011 - 13:37 -- Patrick Sampao
Author(s): 
Hans-Peter Fuehrer, Markus A. Fally, Verena E. Habler, Peter Starzengruber, Paul Swoboda, and Harald Noedl
Reference: 
J. Clin. Microbiol., Apr 2011; 49: 1628 - 1630.

Direct PCR provides a rapid, highly sensitive, and cost-effective alternative to diagnosing malaria using filter paper samples and standard nested PCR.

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Introducing malaria rapid diagnostic tests at registered drug shops in Uganda: Limitations of diagnostic testing in the reality of diagnosis

March 31, 2011 - 12:40 -- Patrick Sampao
Author(s): 
Clare I.R. Chandler, Rachel Hall-Clifford, Turinde Asaph, Magnussen Pascal, Siân Clarke, Anthony K. Mbonye
Reference: 
Social Science & Medicine, Volume 72, Issue 6, March 2011, Pages 937-944

In May to July 2009, we interviewed 9 drug shop workers, 5 health workers and 4 district health officials and carried out 10 focus group discussions with a total of 75 community members to investigate the role of drug shops and the potential for implementation of RDTs at these health care outlets.

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Short Reports: Tales from the bench: laboratory diagnosis of malaria

March 22, 2011 - 09:37 -- Kabogo Ndegwa
Author(s): 
Hilary Edwards
Reference: 
Tropical Doctor, Volume 41, Number 2 Pp. 106-107

This is a practical laboratory overview of current detection methods for malaria parasites, suitable for use in developing countries.

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Open Access | Review: Automated haematology analysis to diagnose malaria

December 2, 2010 - 06:32 -- Kabogo Ndegwa
Author(s): 
Campuzano-Zuluaga G, Hanscheid T, Grobusch MP
Reference: 
Malaria Journal 2010, 9:346 (30 November 2010)

For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis.

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Open Access | Withholding Antimalarials in Febrile Children Who Have a Negative Result for a Rapid Diagnostic Test

August 6, 2010 - 08:30 -- Kabogo Ndegwa
Author(s): 
Valérie d’Acremont, Aggrey Malila, Ndeniria Swai, Robert Tillya, Judith Kahama‐Maro, Christian Lengeler, and Blaise Genton
Reference: 
Clinical Infectious Diseases 1 September 2010, Vol. 51, No. 5: 506-511

Not giving antimalarial drugs in febrile children who had a negative RDTm result was safe, even in an area highly endemic for malaria. Our study provides evidence for treatment recommendations based on parasitological diagnosis in children <5 years old.

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Parasitology: Evaluation of a Loop-Mediated Isothermal Amplification Method as a Tool for Diagnosis of Infection by the Zoonotic Simian Malaria Parasite Plasmodium knowlesi

July 16, 2010 - 12:11 -- Patrick Sampao
Author(s): 
Hiroshi Iseki, Satoru Kawai, Nobuyuki Takahashi, Makoto Hirai, Kazuyuki Tanabe, Naoaki Yokoyama, and Ikuo Igarashi
Reference: 
J. Clin. Microbiol., Jul 2010; 48: 2509 - 2514

In the present study, we developed a LAMP method for the molecular diagnosis of Plasmodium knowlesi infection (PkLAMP) and evaluated its sensitivity, specificity, and clinical applicability.

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Comparative Diagnosis of Malaria Infections by Microscopy, Nested PCR, and LAMP in Northern Thailand

July 2, 2010 - 12:54 -- Patrick Sampao
Author(s): 
Birgit Pöschl, Jarurin Waneesorn, Oriel Thekisoe, Salakchit Chutipongvivate, AND Karanis Panagiotis
Reference: 
Am J Trop Med Hyg, Jul 2010; 83: 56 - 60.

This study further validates LAMP as an alternative molecular diagnostic tool, which can be used in the diagnosis of early infections of malaria cases and together with nPCR can also be used as supplementary methods for clinical and epidemiological use.

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Evaluation of rapid diagnostic tests for malaria case management in Gabon

November 13, 2009 - 15:39 -- Ingeborg van Schayk
Author(s): 
Denise Patricia Mawili-Mboumba, Marielle Karine Bouyou Akotet, Edgard Brice Ngoungou and Maryvonne Kombila
Reference: 
Diagnostic Microbiology and Infectious Disease, Article in Press, Corrected Proof

 

These tests should be considered as a good alternative to microscopy, allowing not only an efficient and rapid diagnosis of malaria in primary health facilities but also to aid in promoting changes for antimalarial prescription behavior.

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Glucose-6-phosphate Dehydrogenase Deficiency and Malaria: Cytochemical Detection of Heterozygous G6PD Deficiency in Women

October 25, 2009 - 16:51 -- Bart G.J. Knols
Author(s): 
Anna L. Peters and Cornelis J.F. Van Noorden
Reference: 
J. Histochem. Cytochem. 2009; 57:1003-1011

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a X-chromosomally transmitted disorder of the erythrocyte that affects 400 million people worldwide. Diagnosis of heterozygously-deficient women is complicated: as a result of lyonization, these women have a normal and a G6PD-deficient population of erythrocytes. The cytochemical assay is the only reliable assay to discriminate between heterozygously-deficient women and non-deficient women or homozygously-deficient women. G6PD deficiency is mainly found in areas where malaria is or has been endemic.

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