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rapid diagnostic tests (RDTs)

Non-invasive surveillance of Plasmodium infection by real-time PCR analysis of ethanol preserved faeces from Ugandan school children with intestinal schistosomiasis

April 4, 2019 - 15:36 -- Open Access
Author(s): 
Hajri Al-Shehri, B. Joanne Power, J. Russell Stothard, et al.
Reference: 
Malaria Journal 2019 18:109, 1 April 2019

In this high transmission setting, EPF sampling with rtPCR analysis has satisfactory diagnostic performance in estimation of mean prevalence and prevalence by school upon direct comparison with POC-RDTs.

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In vivo compartmental kinetics of Plasmodium falciparum histidine-rich protein II in the blood of humans and in BALB/c mice infected with a transgenic Plasmodium berghei parasite expressing histidine-rich protein II

March 13, 2019 - 14:44 -- Open Access
Author(s): 
Kristin E. Poti, Amanda E. Balaban, Priya Pal, Tamaki Kobayashi, Daniel E. Goldberg, Photini Sinnis and David J. Sullivan
Reference: 
Malaria Journal 2019 18:78, 13 March 2019

The data suggest that persistence of PfHRP2 is due to slower clearance of protein from the RBC fraction of the whole blood.

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On the discriminatory and predictive accuracy of the RDT against the microscopy in the diagnosis of malaria among under-five children in Nigeria

February 26, 2019 - 15:16 -- Open Access
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Author(s): 
Adeniyi Francis Fagbamigbe
Reference: 
Malaria Journal 2019 18:46, 21 February 2019

Although there was a significant agreement in the outcomes of RDT and microscopy tests, the discriminatory accuracy of RDT was weak.

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Collaborative intelligence and gamification for on-line malaria species differentiation

January 27, 2019 - 15:03 -- Open Access
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Author(s): 
María Linares, María Postigo, Miguel Luengo-Oroz, et al.
Reference: 
Malaria Journal 2019 18:21, 24 January 2019

These findings show that it is possible to train malaria-naïve non-experts to identify and differentiate malaria species in digitalized thin blood samples.

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Accuracy of One Step malaria rapid diagnostic test (RDT) in detecting Plasmodium falciparum placental malaria infection in women living in Yaoundé, Cameroon

December 5, 2018 - 15:53 -- Open Access
Author(s): 
Rosette Megnekou, Jean Claude Djontu, Benderli C. Nana, Jude D. Bigoga, Maurice Fotso, Balotin Fogang and Rose F. G. Leke
Reference: 
Malaria Journal 2018 17:450, 4 December 2018

These results depict One Step malaria HRP-II RDT to be better in detecting placental P. falciparum infection in pregnant women compared to Giemsa-stained peripheral thick blood smear.

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A new highly sensitive enzyme-linked immunosorbent assay for the detection of Plasmodium falciparum histidine-rich protein 2 in whole blood

November 6, 2018 - 14:04 -- Open Access
Author(s): 
Ihn Kyung Jang, Smita Das, Gonzalo J. Domingo, et al.
Reference: 
Malaria Journal 2018 17:403, 1 November 2018

The HS ELISA is a robust and reproducible assay. The findings suggest that the HS ELISA may be a useful tool as an affordable reference assay for new ultra-sensitive HRP2-based RDTs.

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Can malaria rapid diagnostic tests by drug sellers under field conditions classify children 5 years old or less with or without Plasmodium falciparum malaria? Comparison with nested PCR analysis

October 17, 2018 - 15:16 -- Open Access
Author(s): 
Freddy Eric Kitutu, Henry Wamani, Katarina Ekholm Selling, Fred Ashaba Katabazi, Ronald Bisaso Kuteesa, Stefan Peterson, Joan Nakayaga Kalyango and Andreas Mårtensson
Reference: 
Malaria Journal 2018 17:365, 16 October 2018

Drug sellers can use RDTs in field conditions and achieve acceptable accuracy for malaria diagnosis, and they comply with the RDT results.

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Evaluation of loop-mediated isothermal amplification as a surveillance tool for malaria in reactive case detection moving towards elimination

July 14, 2018 - 15:12 -- Open Access
Author(s): 
Munyaradzi Tambo, Joyce R. Auala, Hugh J. Sturrock, Immo Kleinschmidt, Ronnie Bock, Jennifer L. Smith, Roland Gosling and Davis R. Mumbengegwi
Reference: 
Malaria Journal 2018 17:255, 9 July 2018

The study showed that LAMP had the equivalent performance as nPCR for the identification of Plasmodium falciparum infection.

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Comparison of glucose-6 phosphate dehydrogenase status by fluorescent spot test and rapid diagnostic test in Lao PDR and Cambodia

June 26, 2018 - 16:28 -- Open Access
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Author(s): 
Gisela Henriques, Koukeo Phommasone, Mayfong Mayxay, et al.
Reference: 
Malaria Journal 2018 17:243, 22 June 2018

The interpretation of RDT results requires some training but is a good alternative to the FST.

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How long do rapid diagnostic tests remain positive after anti-malarial treatment?

June 13, 2018 - 08:29 -- Open Access
Author(s): 
Ursula Dalrymple, Rohan Arambepola, Peter W. Gething and Ewan Cameron
Reference: 
Malaria Journal 2018 17:228, 8 June 2018

RDTs remain positive for a highly variable amount of time after treatment with anti-malarials, and the duration of positivity is highly dependent on the type of RDT used for diagnosis.

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