In this high transmission setting, EPF sampling with rtPCR analysis has satisfactory diagnostic performance in estimation of mean prevalence and prevalence by school upon direct comparison with POC-RDTs.
rapid diagnostic tests (RDTs)
The data suggest that persistence of PfHRP2 is due to slower clearance of protein from the RBC fraction of the whole blood.
Although there was a significant agreement in the outcomes of RDT and microscopy tests, the discriminatory accuracy of RDT was weak.
These findings show that it is possible to train malaria-naïve non-experts to identify and differentiate malaria species in digitalized thin blood samples.
These results depict One Step malaria HRP-II RDT to be better in detecting placental P. falciparum infection in pregnant women compared to Giemsa-stained peripheral thick blood smear.
The HS ELISA is a robust and reproducible assay. The findings suggest that the HS ELISA may be a useful tool as an affordable reference assay for new ultra-sensitive HRP2-based RDTs.
Drug sellers can use RDTs in field conditions and achieve acceptable accuracy for malaria diagnosis, and they comply with the RDT results.
The study showed that LAMP had the equivalent performance as nPCR for the identification of Plasmodium falciparum infection.
The interpretation of RDT results requires some training but is a good alternative to the FST.
RDTs remain positive for a highly variable amount of time after treatment with anti-malarials, and the duration of positivity is highly dependent on the type of RDT used for diagnosis.