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PCR

Candidate Human Genetic Polymorphisms and Severe Malaria in a Tanzanian Population

November 11, 2012 - 12:29 -- Open Access
Author(s): 
Alphaxard Manjurano, Taane G. Clark, Christopher Drakeley , et al.
Reference: 
PLoS ONE 7(10): e47463
MalariaWorld

Human genetic background strongly influences susceptibility to malaria infection and progression to severe disease and death.

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Short Technical Report: Effect of PCR extension temperature on high-throughput sequencing

January 25, 2011 - 07:13 -- Patrick Sampao
Author(s): 
María José López-Barragán, Mariam Quiñones, Kairong Cui, Jacob Lemieux, Keji Zhao, Xin-zhuan Su
Reference: 
Molecular and Biochemical Parasitology, Volume 176, Issue 1, March 2011, Pages 64-67

Here we compare genome-wide nucleosome coverage in libraries amplified at three different extension temperatures and show that reduction in PCR extension temperature from 70 °C to 60 °C can greatly increase the fraction of coverage at AT-rich regions of the P. falciparum genome. Our method will improve the efficiency and coverage in sequencing an AT-rich genome.

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Post-Arrival Screening for Malaria in Asymptomatic Refugees Using Real-Time PCR

January 7, 2011 - 11:37 -- Patrick Sampao
Author(s): 
Chelsea E. Matisz., Prenilla Naidu., Stephanie K. Yanow., et al.
Reference: 
Am J Trop Med Hyg, Jan 2011; 84: 161 - 165.

These results suggest that appropriate guidelines for malaria screening should consider the risk of relapsing infections, and they highlight the potential usefulness of real-time PCR in the diagnosis of asymptomatic malaria.

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Short Communication: DNA sequencing of 13 cytokine gene fragments of Aotus infulatus and Saimiri sciureus, two non-human primate models for malaria

November 9, 2010 - 08:01 -- Kabogo Ndegwa
Author(s): 
F.A. Alves, M.T. Souza, L.J.M. Carvalhoa et al.
Reference: 
Cytokine, Volume 52, Issue 3, December 2010, Pages 151-155

Aotus and Saimiri are non-human primate models recommended by the World Health Organization for experimental studies in malaria, especially for vaccine pre-clinical trials.

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Short report: Malaria Diagnosis by a Polymerase Chain Reaction–Based Assay Using a Pooling Strategy

November 10, 2009 - 21:04 -- Ingeborg van Schayk
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Author(s): 
Ajay R. Bharti, Scott L. Letendre, Kailash P. Patra, Joseph M. Vinetz, AND Davey M. Smith
Reference: 
Am J Trop Med Hyg 2009;81 754-757

Pooling clinical specimens reduces the number of assays needed when screening for infectious diseases. Polymerase chain reaction (PCR)-based assays are the most sensitive tests to diagnose malaria, but its high cost limits its use.

 

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