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proteins

Short Technical Report: A densely overlapping gene fragmentation approach improves yeast two-hybrid screens for Plasmodium falciparum proteins

May 25, 2011 - 14:47 -- Patrick Sampao
Author(s): 
Hakeenah F. Brown, Ling Wang, Sudip Khadka, Stanley Fields, Douglas J. LaCount
Reference: 
Molecular and Biochemical Parasitology, Volume 178, Issues 1-2, July-August 2011, Pages 56-59

We report here two methods to create gene fragments – random fragmentation by partial DNAse I digestion and generation of densely overlapping fragments by PCR – that enable most portions of P. falciparum genes to be expressed and screened in the yeast two-hybrid assay. The PCR-based method is less technically challenging and facilitates fine-scale mapping of protein interaction domains.

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Short Communication: Low genetic polymorphism of merozoite surface proteins 7 and 10 in Colombian Plasmodium vivax isolates

March 1, 2011 - 08:01 -- Patrick Sampao
Author(s): 
Diego Garzón-Ospina, Liza Romero-Murillo, Luisa F. Tobón, Manuel A. Patarroyo
Reference: 
Infection, Genetics and Evolution, Volume 11, Issue 2, March 2011, Pages 528-531

Considering that antigens included in a subunit malaria vaccine should be both accessible to the immune system and lack genetic diversity or have very limited polymorphism, we have analyzed the genetic diversity of three msp genes (msp-7A, msp-7K and msp-10) in different geographical regions of Colombia. The results showed that these genes follow the neutral model of evolution and also display low genetic diversity.

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Open Access | Malaria IMC1 Membrane Skeleton Proteins Operate Autonomously and Participate in Motility Independently of Cell Shape

February 15, 2011 - 12:04 -- Patrick Sampao
Author(s): 
Annie Z. Tremp and ,Johannes T. Dessens
Reference: 
The Journal of Biological Chemistry, February 18, 2011 286, 5383-5391.

We show here that a third family member, IMC1h, has a distinct differential expression pattern and localizes to the pellicle of both ookinetes and sporozoites. Knock-out of IMC1h mimics the loss-of-function phenotypes of IMC1a and IMC1b in their respective life stages, indicating that IMC1 proteins could be operating co-dependently.

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Open Access | Oral Presentation: A library of functional recombinant Plasmodium falciparum merozoite surface proteins

November 5, 2010 - 11:29 -- Patrick Sampao
Author(s): 
Cecile Crosnier, Madushi Wanaguru, Brian McDade, Julian Rayner, Gavin J Wright
Reference: 
Malaria Journal 2010, 9(Suppl 2):O8 (20 October 2010)

All the clinical symptoms of malaria result from the invasion of human erythrocytes by the parasite's merozoites.

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Parasite-encoded Hsp40 proteins define novel mobile structures in the cytosol of the P. falciparum-infected erythrocyte

September 14, 2010 - 07:31 -- Patrick Sampao
Author(s): 
Simone K., Melanie R., et al.
Reference: 
Cellular Microbiology, Vol 12, Issue 10, pg 1398–1420, Oct 2010

Here, we have generated transfectant lines expressing GFP- or HA-Strep-tagged versions of these proteins, and used these to investigate both localization and other properties of these Hsp40 co-chaperones.

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Sequence variation of ookinete surface proteins Pvs25 and Pvs28 of Plasmodium vivax isolates from Southern Mexico and their association to local anophelines infectivity

May 27, 2010 - 11:50 -- Patrick Sampao
Author(s): 
Lilia C., Alejandro D., et al.
Reference: 
Infection, Genetics and Evolution, Volume 10, Issue 5, July 2010, Pages 645-654

The polymorphism of Pvs25 and Pvs28 ookinete surface proteins, their association to circumsporozoite protein repeat (CSPr) genotypes (Vk210 and Vk247) and their infectivity to local Anopheles albimanus and Anopheles pseudopunctipennis were investigated in Plasmodium vivax-infected blood samples obtained from patients in Southern Mexico.

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