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DNA

Open Access | Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples

August 24, 2011 - 09:27 -- Kabogo Ndegwa
Author(s): 
Taylor BJ, Martin KA, Arango E, Agudelo OM, Maestre A, Yanow SK
Reference: 
Malaria Journal 2011, 10:244 (19 August 2011)

The methodology described facilitates high-throughput testing of blood samples collected in the field by fluorescence-based real-time PCR.

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Plasmodium falciparum: DNA sequence specificity of cisplatin and cisplatin analogues

July 25, 2011 - 08:09 -- Kabogo Ndegwa
Author(s): 
Vincent Murray, Heather M. Campbell, Annette M. Gero
Reference: 
Experimental Parasitology, Volume 128, Issue 4, August 2011, Pages 396-400

In this paper, we provided evidence that cisplatin is able to form adducts with cellular DNA in Plasmodium falciparum.

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Open Access | Investigating the Host Binding Signature on the Plasmodium falciparum PfEMP1 Protein Family

May 10, 2011 - 07:52 -- Kabogo Ndegwa
Author(s): 
Joel H. Janes, Christopher P. Wang, Emily Levin-Edens, Inès Vigan-Womas, Micheline Guillotte, Martin Melcher, Odile Mercereau-Puijalon, Joseph D. Smith
Reference: 
PLoS Pathog 7(5): e1002028

Recombination has an evident impact on virus evolution and emergence of new pathotypes, and has generated an immense literature.

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Short Communication: Malaria drug resistance is associated with defective DNA mismatch repair

April 11, 2011 - 14:04 -- Patrick Sampao
Author(s): 
Meryl A. Castellini, Jeffrey S. Buguliskis, Louis J. Casta, Charles E. Butz, Alan B. Clark, Thomas A. Kunkel, Theodore F. Taraschi
Reference: 
Molecular and Biochemical Parasitology, Volume 177, Issue 2, June 2011, Pages 143-147

These data imply that antimalarial drug resistance can result from defective MMR.

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Open Access | Measuring the efficacy of anti-malarial drugs in vivo: quantitative PCR measurement of parasite clearance

November 16, 2010 - 11:58 -- Kabogo Ndegwa
Author(s): 
Khalid B Beshir, Rachel L Hallett, Alice C Eziefula, Robin Bailey, Julie Watson, Stephen G Wright, Peter L Chiodini, Spencer D Polley, Colin J Sutherland
Reference: 
Malaria Journal 2010, 9:312 (5 November 2010)

Large studies, particularly in Africa where children represent a major proportion of treated cases, will require a simpler blood sample collection regime, and a method capable of high throughput.

Prime-boost vectored malaria vaccines: Progress and prospects

October 25, 2009 - 16:25 -- Ingeborg van Schayk
Author(s): 
Adrian V. S. Hill, Arturo Reyes-Sandoval, Geraldine O’Hara, Katie Ewer, Alison Lawrie, Anna Goodman, Alfredo Nicosia, Antonella Folgori, Stefano Colloca, Riccardo Cortese, Sarah C. Gilbert and Simon Draper
Reference: 
Human Vaccines, Volume 6, Issue 1, January 2010

The difficulty of inducing protective immunity through antibodies against sporozoites led to efforts to assess vectored vaccines as a means of inducing protective T cell immunity against the malaria liver-stage parasite. Although DNA vectored vaccines used alone were poorly immunogenic and not protective, high levels of parasite clearance in the liver has been achieved with viral vectored vaccines used in heterologous prime-boost regimes.

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