This ELISA-based in vitro drug assay is easy to implement, fast, and avoids the use radioisotopes or expensive equipment.
The emergence of highly chloroquine (CQ) resistant P. vivax in Southeast Asia has created an urgent need for an improved understanding of the mechanisms of drug resistance in these parasites, the development of robust tools for defining the spread of resistance, and the discovery of new antimalarial agents.
No abstract available
There is an urgent need for new antimalarial drugs with novel mechanisms of action to deliver effective control and eradication programs.
Despite efforts to reduce malaria morbidity and mortality, drug-resistant parasites continue to evade control strategies.
The work described here represents another significant step towards determination of the activity of new molecules on mature gametocytes of any strain with an automated assay suitable for medium/high-throughput screening.
Reversing the spread of HIV infection and the incidence of malaria constitute two of the Millenium Development Goals.
Nitidine can be considered a potential anti-malarial lead compound. Its ability to complex haem and inhibit beta-haematin formation suggests a mechanism of action similar to that of chloroquine.
These data enable objective comparisons of the strengths and weaknesses of each chemical class at targeting each stage of the lifecycle.
Chemotherapy is still the cornerstone for malaria control. Developing drugs against Plasmodium parasites and monitoring their efficacy requires methods to accurately determine the parasite killing rate in response to treatment.