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Gal4 transactivators

Optimization of the Gal4-UAS system in an Anopheles gambiae cell line

September 20, 2011 - 13:22 -- Patrick Sampao
Author(s): 
A. Lynd, G. J. Lycett
Reference: 
Insect Molecular Biology, Volume 20, Issue 5, pages 599–608, October 2011

The results indicated that the native Gal4 is only weakly active in these cells. Modified forms of Gal4, including those carrying minimal VP16 activation domains, as well as a deleted form of Gal4, give up to 20-fold greater activity than the native protein, when used in conjunction with a responder plasmid having 14 UAS repeats.

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