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SYBR Green I

Antimalarial Drug Sensitivity Profile of Western Kenya Plasmodium falciparum Field Isolates Determined by a SYBR Green I in vitro Assay and Molecular Analysis

July 8, 2011 - 09:02 -- Patrick Sampao
Author(s): 
Hoseah M. Akala., Fredrick L. Eyase., Douglas S. Walsh., et al
Reference: 
Am J Trop Med Hyg 2011 vol. 85 no. 1 34-41

In 2007–2008, we assayed 121 P. falciparum field isolates from western Kenya for 50% inhibitory concentrations (IC50) against 6 antimalarial drugs using a SYBR Green I in vitro assay: 91 immediate ex vivo (IEV) and 30 culture-adapted, along with P. falciparum reference clones D6 (chloroquine [CQ] sensitive) and W2 (CQ resistant).

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