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cyclophilins

Overexpression, purification and assessment of cyclosporin binding of a family of cyclophilins and cyclophilin-like proteins of the human malarial parasite Plasmodium falciparum

June 13, 2011 - 14:14 -- Patrick Sampao
Author(s): 
Alejandro Marín-Menéndez, Angus Bell
Reference: 
Protein Expression and Purification, Volume 78, Issue 2, August 2011, Pages 225-234

Previously a pET vector conferring a C-terminal His6 tag was used for recombinant expression and purification of one member of the P. falciparum cyclophilin family in Escherichia coli. The approach here was to use an identical method to produce all of the other members of this family and thereby allow the most consistent functional comparisons.

Medical Treatment: 
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