The present study found that the recombinant Trx–tHAP fusion protein aggregated during purification which could be prevented through the addition of 0.2% CHAPS. Trx–tHAP fusion protein was processed into a mature form of tHAP (mtHAP) by both autoactivation, and activation with either enterokinase or plasmepsin II.
Molecular and Biochemical Parasitology, Volume 173, Issue 1, September 2010, Pages 17-24
Molecular and Biochemical Parasitology, Volume 171, Issue 1, May 2010, Pages 25-31
Here, we generate parasite lines expressing HT motif containing proteins that are localized to different compartments within the parasite or host cell.