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Positive Selection of Plasmodium falciparum Parasites With Multiple var2csa-Type PfEMP1 Genes During the Course of Infection in Pregnant Women

May 19, 2011 - 09:35 -- Patrick Sampao
Adam F. Sander, Ali Salanti, Thomas Lavstsen, Morten A. Nielsen, Thor G. Theander, Rose G. F. Leke, Yeung Y. Lo, Naveen Bobbili, David E. Arnot and Diane W. Taylor
J Infect Dis. (2011) 203 (11): 1679-1685.

The data suggest that multiplicity of var2csa-type genes enables P. falciparum parasites to persist for a longer period of time during placental infections, probably because of their greater capacity for antigenic variation and evasion of variant-specific immune responses.

Open Access | Surface Co-Expression of Two Different PfEMP1 Antigens on Single Plasmodium falciparum-Infected Erythrocytes Facilitates Binding to ICAM1 and PECAM1

September 9, 2010 - 12:07 -- Patrick Sampao
Louise J., Dominique C., et al.
PLoS Pathogens Volume 6 Issue 9 September 02, 2010

These new results on PfEMP1 antigen expression indicate that a re-evaluation of the molecular mechanisms involved in P. falciparum adhesion and of the accepted paradigm of absolutely mutually exclusive var gene transcription is required.

Open Access | The kinetics of antibody binding to Plasmodium falciparum VAR2CSA PfEMP1 antigen and modelling of PfEMP1 antigen packing on the membrane knobs

April 20, 2010 - 09:29 -- Kabogo Ndegwa
Joergensen LM, Salanti A, Dobrilovic T, Barfod L, Hassenkam T, Theander TG, Hviid L, Arnot DE
Malaria Journal 2010, 9:100 (19 April 2010)

High avidity is required to achieve the strongest binding to VAR2CSA PfEMP1, but the structures that display PfEMP1 also tend to inhibit cross-linking between PfEMP1 antigens, by holding many binding epitopes at distances beyond the 15-18nm sweep radius of an antibody.

Biochemical and biophysical characterisation of DBL1α1-varO, the rosetting domain of PfEMP1 from the VarO line of Plasmodium falciparum

February 11, 2010 - 09:04 -- Kabogo Ndegwa
Alexandre Juillerata, Sébastien Igoneta, Inès Vigan-Womas, Micheline Guillotte, Stéphane Gangnard, Grazyna Faure, Bruno Baron, Bertrand Raynal, Odile Mercereau-Puijalon and Graham A. Bentley
Molecular and Biochemical Parasitology, Volume 170, Issue 2, April 2010, Pages 84-92

Rosetting of erythrocytes infected with Plasmodium falciparum is frequently observed in children with severe malaria. This adhesion phenomenon has been linked to the DBL1α domain of P. falciparum erythrocyte membrane protein 1 (PfEMP1) in three laboratory clones: FCR3S1.2, IT4R29 and Palo Alto varO.

In vitro selection of RNA aptamers against a conserved region of the Plasmodium falciparum erythrocyte membrane protein 1

October 29, 2009 - 14:18 -- Ingeborg van Schayk
Anders Barfod, Tina Persson & Johan Lindh
Parasitology Research, Volume 105, Number 6 / November, 2009 : 1557-1566, DOI 10.1007/s00436-009-1583-x

In this study we use Systematic Evolution of Ligands by EXponential enrichment (SELEX) methodology to isolate high-affinity ligands (aptamers). To validate the results from the SELEX in vitro selection, different aptamers have been selected against PfEMP1 in a live cell assay of P. falciparum strain FCR3S1.2, a highly rosetting strain.

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