Our analysis showed that the capacity of innate responses to restrict initial parasite growth saturates with parasite dose and that experimentally enhanced innate immunity can affect parasite density indirectly via resource depletion.
Drawing on data from mice, they present a new statistical approach to analyzing how parasitemia changes over time and to quantifying and comparing the roles played by the immune system and the availability of red blood cells in regulating parasite numbers.
A genetic dissection approach was employed to determine whether the IL-2 receptor complex (IL-2R) comprised of α, β and γ chains is required for the suppression of Plasmodium chabaudi adami parasitemia.
Plasmodium yoelii is an excellent model for studying malaria pathogenesis that is often intractable to investigate using human parasites;
Recombinant Plasmodium falciparum merozoite surface protein 3 (PfMSP3F) and a 24-kDa fragment from its N terminus (MSP3N) that includes the essential conserved domain, which elicits the maximum antibody (Ab)-dependent cellular inhibition (ADCI), were expressed as soluble proteins in Escherichia coli. Both proteins were found to be stable in both soluble and lyophilized forms.
The absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy.
Evidence is accumulating that miRNAs are critically implicated in the outcome of diseases, but little information is available for infectious diseases.
To mimic a human malaria infection in the endemic condition, two strains of mice (Balb/c and CBA) were infected and treated several times to generate so-called semi-immune status.
Although the storage of asexual blood-stage P. falciparum parasites at 4°C is detrimental to their survival (a 7.1-fold reduction in parasitemia after 14 days in storage), parasites remained microscopically detectable for 28 days, the end time point of our study.
Studies in malaria patients indicate that higher frequencies of peripheral blood CD4+ Foxp3+ CD25+ regulatory T (Treg) cells correlate with increased blood parasitemia.