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Anopheles stephensi

Evaluation of indigenous plant extracts against the malarial vector, Anopheles stephensi (Liston) (Diptera: Culicidae)

June 23, 2011 - 06:43 -- Kabogo Ndegwa
Marimuthu Govindarajan
Parasitology Research, Volume 109, Number 1, 93-103

Since ancient times, plant and microbial products were used in various aspects. However, their use against insects decreased when chemical products became developed.

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Open Access | Engineered Resistance to Plasmodium falciparum Development in Transgenic Anopheles stephensi

May 4, 2011 - 06:30 -- Kabogo Ndegwa
Alison T. Isaacs, Fengwu Li, Nijole Jasinskiene, Xiaoguang Chen, Xavier Nirmala, Osvaldo Marinotti, Joseph M. Vinetz, Anthony A. James
PLoS Pathog 7(4): e1002017

Transposon-mediated transformation was used to produce Anopheles stephensi that express single-chain antibodies (scFvs) designed to target the human malaria parasite, Plasmodium falciparum.

Open Access | Knockdown resistance (kdr)-like mutations in the voltage-gated sodium channel of a malaria vector Anopheles stephensi and PCR assays for their detection

March 15, 2011 - 08:43 -- Kabogo Ndegwa
Singh OP, Dykes CL, Lather M, Agrawal OP, Adak T
Malaria Journal 2011, 10:59 (14 March 2011)

Two alternative kdr-like mutations, L1014S and L1014F, were detected in An. stephensi with a high allelic frequency of L1014S.

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Open Access | Analysis of Two Novel Midgut-Specific Promoters Driving Transgene Expression in Anopheles stephensi Mosquitoes

February 9, 2011 - 08:54 -- Kabogo Ndegwa
Tony Nolan, Elisa Petris, Hans-Michael Müller, Ann Cronin, Flaminia Catteruccia, Andrea Crisanti
PLoS ONE 6(2): e16471

Our studies on two putative blood-meal induced, midgut-specific promoters validate the use of G12 upstream regulatory regions to drive targeted transgene expression coinciding spatially and temporally with pre-sporogonic stages of Plasmodium parasites in the mosquito, offering the possibility of manipulating vector competence or performing functional studies on vector-parasite interactions.

Open Access | The role of proboscis of the malaria vector mosquito Anopheles stephensi in host-seeking behavior

January 31, 2011 - 09:57 -- Patrick Sampao
Maekawa E, Aonuma H, Nelson B, Yoshimura A, Tokunaga F, Fukumoto S, Kanuka H
Parasites & Vectors 2011, 4:10 (27 January 2011)

Our findings support an emerging view with a possible role of proboscis as important equipment during host-seeking, and give us an insight into how these appendages likely evolved from a common origin in order to function as antenna organs.

Open Access | The Function and Three-Dimensional Structure of a Thromboxane A2/Cysteinyl Leukotriene-Binding Protein from the Saliva of a Mosquito Vector of the Malaria Parasite

December 2, 2010 - 06:03 -- Kabogo Ndegwa
Patricia H. Alvarenga, Ivo M. B. Francischetti, Eric Calvo, Anderson Sá-Nunes, José M. C. Ribeiro, John F. Andersen
PLoS Biol 8(11): e1000547

The highly expressed D7 protein family of mosquito saliva has previously been shown to act as an anti-inflammatory mediator by binding host biogenic amines and cysteinyl leukotrienes (CysLTs).

Open Access | Development of larval thermotolerance and its impact on adult susceptibility to malathion insecticide and Plasmodium vivax infection in Anopheles stephensi

November 19, 2010 - 11:18 -- Kabogo Ndegwa
Kamaraju Raghavendra, Tapan K. Barik & Tridibes Adak
Parasitology Research, Volume 107, Number 6, Pages 1291-1297

The effect of the range of temperature on the thermal adaptation in Anopheles stephensi Liston 1901 was evaluated in the laboratory. Late third instar larvae of An. stephensi were exposed to variable temperatures viz. 37°C, 39°C, 41°C, 43°C and 45°C, and their lethal time to cause 50% mortality (LTM50) values were calculated.

Open Access | Population biology of malaria within the mosquito: density-dependent processes and potential implications for transmission-blocking interventions

November 16, 2010 - 11:53 -- Kabogo Ndegwa
Thomas S Churcher, Emma J Dawes, Robert E Sinden, George K Christophides, Jacob C Koella, Maria-Gloria Basanez
Malaria Journal 2010, 9:311 (4 November 2010)

A greater understanding of the development of the malaria parasite within the mosquito is required to fully evaluate the impact of TBIs.

Open Access | Oral Presentation: Characterization and comparative sequence analyzes of GABA receptor gene in Asian main malaria mosquito, Anopheles stephensi

November 8, 2010 - 09:26 -- Patrick Sampao
Saber Gholizadeh, Ameneh Karimi, Nooshin Bayat, Navid Dinparast Djadid, Sedigheh Zakeri
Malaria Journal 2010, 9(Suppl 2):O15 (20 October 2010)

Here we report the sequence analysis of the Rdl gene from Asian main malaria mosquito, Anopheles stephensi, using specific primers in the polymerase chain reaction.


A Physical Map for an Asian Malaria Mosquito, Anopheles stephensi

November 1, 2010 - 13:58 -- Patrick Sampao
Maria V. Sharakhova, Ai Xia, Zhijian Tu, Yogesh S. Shouche, Maria F. Unger, AND Igor V. Sharakhov
Am J Trop Med Hyg, Nov 2010; 83: 1023 - 1027.

Physical mapping is a useful approach for studying genome organization and evolution as well as for genome sequence assembly.



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