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amplification

Spurious Amplification of a Plasmodium vivax Small-Subunit RNA Gene by Use of Primers Currently Used To Detect P. knowlesi

December 22, 2009 - 13:32 -- Kabogo Ndegwa
Author(s): 
Mallika Imwong, Naowarat Tanomsing, Sasithon Pukrittayakamee, Nicholas P. J. Day, Nicholas J. White and Georges Snounou
Reference: 
Journal of Clinical Microbiology, December 2009, p. 4173-4175, Vol. 47, No. 12

The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes.

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