A more in-depth understanding of local ecological factors influencing malaria disease as shown in present study may not only be useful for developing sustainable regional malaria control programmes, but can also benefit malaria elimination efforts at village level.
Different trapping methods were suitable for different species, likely reflecting differences in behaviour among species
The results indicated that although we did not find any microscopically slide positive cases, six PCR positive subjects were found.
The species distributions of Anopheles mosquitoes attracted to human, goat, and cow hosts were similar.
We find that rural Papuans as well as those who live in poor, densely forested, lowland districts are at a higher risk of infection than their counterparts. We also find age and gender differentials in malaria prevalence, if only to a small degree.
This study confirmed a zoonotic infection using polymerase chain reaction amplification and Sanger sequencing of plasmodial DNA encoding the mitochondrial cytochrome c oxidase subunit I (mtCOI).
The diagnostic accuracy to detect malaria among mostly asymptomatic pregnant women and perceived ease of use was slightly better with First-Response ® , but overall, differences between the four RDTs were small and performance comparable to field microscopy.
With proper malaria diagnosis, SP may still be used as a rational anti-malarial drug either as a single prescription or in combination with artemisinin.
A new malaria elimination approach that fits the local disease transmission, intervention and political system is required.
The study highlights the importance of careful assessment of diagnostic capacity when embarking upon a large-scale malaria elimination programme.