Human malaria is caused by protozoan parasites of the genus Plasmodium (Plasmodium falciparum, P. vivax, P. malariae, P. ovale, and P. knowlesi).
The nested PCR method is more reliable than conventional microscopic examination for the diagnosis of malaria infections, and this is particularly true in cases of mixed infections and submicroscopic infections.
This study demonstrated that Plasmodium species do not share a common mechanism of sporozoite escape, as previously thought, but show complex and species-specific mechanisms.
This study shows that in areas of low transmission in Kinshasa, the peak prevalence occurs in older age groups however ITN use was highest in children under 5 years.
This ultrasensitive RT-PCR method is a robust, field-tested screening method that is vastly more sensitive than RDTs.
The PCR detected more positive samples than the microscopy; in addition, P. ovale and P. ovale/P. vivax were detected that had not been detected by microscopy, which can affect in the infection control.